Journal article
A Role for Sterol Levels in Oxygen Sensing in Saccharomyces cerevisiae
Genetics (Austin), Vol.174(1), pp.191-201
09/2006
DOI: 10.1534/genetics.106.059964
PMCID: PMC1569814
PMID: 16783004
Abstract
Upc2p and Ecm22p are a pair of transcription factors responsible for the basal and induced expression of genes encoding enzymes of ergosterol biosynthesis in yeast (\nERG\ngenes). Upc2p plays a second role as a regulator of hypoxically expressed genes. Both sterols and heme depend upon molecular oxygen for their synthesis, and thus the levels of both have the potential to act as indicators of the oxygen environment of cells. Hap1p is a heme-dependent transcription factor that both Upc2 and Ecm22p depend upon for basal level expression of\nERG\ngenes. However, induction of both\nERG\ngenes and the hypoxically expressed\nDAN/TIR\ngenes by Upc2p and Ecm22p occurred in response to sterol depletion rather than to heme depletion. Indeed, upon sterol depletion, Upc2p no longer required Hap1p to activate\nERG\ngenes. Mot3p, a broadly acting repressor/activator protein, was previously shown to repress\nERG\ngene expression, but the mechanism was unclear. We established that Mot3p bound directly to Ecm22p and repressed Ecm22p- but not Upc2p-mediated gene induction.
Details
- Title: Subtitle
- A Role for Sterol Levels in Oxygen Sensing in Saccharomyces cerevisiae
- Creators
- Brandon S. J Davies - Department of Molecular and Cellular Biology, Division of Genetics, Genomics, and Development, University of California, Berkeley, California 94701-3202Jasper Rine - Department of Molecular and Cellular Biology, Division of Genetics, Genomics, and Development, University of California, Berkeley, California 94701-3202
- Resource Type
- Journal article
- Publication Details
- Genetics (Austin), Vol.174(1), pp.191-201
- DOI
- 10.1534/genetics.106.059964
- PMID
- 16783004
- PMCID
- PMC1569814
- ISSN
- 0016-6731
- eISSN
- 1943-2631
- Language
- English
- Date published
- 09/2006
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology
- Record Identifier
- 9984024403102771
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