Journal article
A Subset of Neutrophils Expressing Markers of Antigen-Presenting Cells in Human Visceral Leishmaniasis
The Journal of infectious diseases, Vol.214(10), pp.1531-1538
11/15/2016
DOI: 10.1093/infdis/jiw394
PMCID: PMC5091370
PMID: 27601622
Abstract
Visceral leishmaniasis (VL) is a potentially fatal parasitic disease associated with fever, cachexia and impaired protective T-cell responses against the parasite.
Peripheral blood leukocytes from 105 subjects with VL and healthy control subjects from the endemic region of Muzaffarpur, Bihar, India, were compared using flow cytometry and reverse-transcriptase quantitative polymerase chain reaction. Findings were correlated with clinical data.
An expanded population of low-density neutrophils that expressed HLA-DR, CD80 and CD86 was observed in subjects with VL. This neutrophil population contracted after successful treatment of disease. Plasma from patients with acute VL was able to induce similar high-level HLA-DR expression in neutrophils from healthy subjects. HLA-DR
neutrophils from subjects with VL did not stimulate T-cell proliferation, but they did express higher programmed cell death ligand-1 (PDL1) than other neutrophils, and lymphocytes of the same subjects expressed high programmed cell death 1 (PD1).
Patients with acute VL have expanded circulating low-density neutrophils expressing markers of antigen presentation, which diminish after treatment. Development of HLA-DR
neutrophils is stimulated, at least in part, by components of plasma from patients with acute disease. Although we found no evidence that they act as antigen-presenting cells, these neutrophils expressed markers implicating a role in T-cell exhaustion.
Details
- Title: Subtitle
- A Subset of Neutrophils Expressing Markers of Antigen-Presenting Cells in Human Visceral Leishmaniasis
- Creators
- Smriti Sharma - Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, IndiaRichard E Davis - Interdisciplinary Program in Immunology, University of IowaShweta Srivastva - Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, IndiaSusanne Nylén - Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, SwedenShyam Sundar - Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, IndiaMary E Wilson - Departments of Internal Medicine and Microbiology, University of Iowa and the Veterans' Affairs Medical Center, Iowa City
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.214(10), pp.1531-1538
- Publisher
- United States
- DOI
- 10.1093/infdis/jiw394
- PMID
- 27601622
- PMCID
- PMC5091370
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Grant note
- P50 AI074321 / NIAID NIH HHS R01 AI045540 / NIAID NIH HHS T32 AI007511 / NIAID NIH HHS R01 AI076233 / NIAID NIH HHS I01 BX001983 / BLRD VA I01 BX000536 / BLRD VA
- Language
- English
- Date published
- 11/15/2016
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Internal Medicine
- Record Identifier
- 9984001205202771
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