Journal article
A Systematic Approach to Identify Markers of Distinctly Activated Human Macrophages
Frontiers in immunology, Vol.6(MAY), pp.253-253
2015
DOI: 10.3389/fimmu.2015.00253
PMCID: PMC4445387
PMID: 26074920
Abstract
Polarization has been a useful concept for describing activated macrophage phenotypes and gene expression profiles. However, macrophage activation status within tumors and other settings are often inferred based on only a few markers. Complicating matters for relevance to human biology, many macrophage activation markers have been best characterized in mice and sometimes are not similarly regulated in human macrophages. To identify novel markers of activated human macrophages, gene expression profiles for human macrophages of a single donor subjected to 33 distinct activating conditions were obtained and a set of putative activation markers were subsequently evaluated in macrophages from multiple donors using integrated fluidic circuit (IFC)-based RT-PCR. Using unsupervised hierarchical clustering of the microarray screen, highly altered transcripts (>4-fold change in expression) sorted the macrophage transcription profiles into two major and 13 minor clusters. Among the 1874 highly altered transcripts, over 100 were uniquely altered in one major or two related minor clusters. IFC PCR-derived data confirmed the microarray results and determined the kinetics of expression of potential macrophage activation markers. Transcripts encoding chemokines, cytokines, and cell surface were prominent in our analyses. The activation markers identified by this study could be used to better characterize tumor-associated macrophages from biopsies as well as other macrophage populations collected from human clinical samples.
Details
- Title: Subtitle
- A Systematic Approach to Identify Markers of Distinctly Activated Human Macrophages
- Creators
- Bayan Sudan - ,Mark A Wacker - ,Mary E Wilson - ,Joel W Graff - ,
- Resource Type
- Journal article
- Publication Details
- Frontiers in immunology, Vol.6(MAY), pp.253-253
- DOI
- 10.3389/fimmu.2015.00253
- PMID
- 26074920
- PMCID
- PMC4445387
- NLM abbreviation
- Front Immunol
- ISSN
- 1664-3224
- eISSN
- 1664-3224
- Publisher
- Frontiers Media S.A
- Grant note
- AI076233; AI045540 / National Institutes of Health Veterans Health Administration 1IK2BX001627; 5I01BX001983; 5I01BX000536 / Department of Veterans Affairs AI007511 / NIH Biomedical Laboratory Research and Development Office of Research and Development
- Language
- English
- Date published
- 2015
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Internal Medicine
- Record Identifier
- 9984001132402771
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