A Transistor-like pH Nanoprobe for Tumour Detection and Image-guided Surgery

Tian Zhao; Gang Huang; Yang Li; Shunchun Yang; Saleh Ramezani; Zhiqiang Lin; Yiguang Wang; Xinpeng Ma; Zhiqun Zeng; Min Luo; Esther de Boer; Xian-Jin Xie; Joel Thibodeaux; Rolf A Brekken; Xiankai Sun; Baran D Sumer; Jinming Gao

doi:10.1038/s41551-016-0006

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A Transistor-like pH Nanoprobe for Tumour Detection and Image-guided Surgery

Journal article

Peer reviewed

A Transistor-like pH Nanoprobe for Tumour Detection and Image-guided Surgery

Tian Zhao, Gang Huang, Yang Li, Shunchun Yang, Saleh Ramezani, Zhiqiang Lin, Yiguang Wang, Xinpeng Ma, Zhiqun Zeng, Min Luo, …

Nature biomedical engineering, Vol.1(1), 6

2016

DOI: 10.1038/s41551-016-0006

PMCID: PMC5617128

PMID: 28966871

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https://research.rug.nl/en/publications/27178c08-ab32-457b-b759-a5585e711736View

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Abstract

Because of profound genetic and histological differences in cancerous tissue, it is challenging to detect a broad range of malignant tumours at high resolution. Here, we report the design and performance of a fluorescent nanoprobe with transistor-like responses (transition pH = 6.9) for the detection of the deregulated pH that drives many of the invasive properties of cancer. The nanoprobe amplifies fluorescence signal in the tumour over that in the surrounding normal tissues, resulting in a discretized, binary output signal with spatial resolution smaller than 1 mm. The nanoprobe allowed us to image a broad range of tumours in mouse models using a variety of clinical cameras, and to perform real-time tumour-acidosis-guided detection and surgery of occult nodules (< 1 mm ) in mice bearing head-and-neck or breast tumours, significantly lengthening mice survivability. We also show that the pH nanoprobe can be used as a reporter in a fast, quantitative assay to screen for tumour-acidosis inhibitors. The binary delineation of pH achieved by the nanoprobe promises to improve the accuracy of cancer detection, surveillance and therapy.

Details

Title: Subtitle: A Transistor-like pH Nanoprobe for Tumour Detection and Image-guided Surgery
Creators: Tian Zhao - Department of Pharmacology, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Gang Huang - Department of Pharmacology, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Yang Li - Department of Pharmacology, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Shunchun Yang - Department of Pharmacology, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Saleh Ramezani - Department of Radiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Zhiqiang Lin - Department of Pharmacology, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Yiguang Wang - Department of Pharmacology, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Xinpeng Ma - Department of Pharmacology, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Zhiqun Zeng - Department of Pharmacology, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Min Luo - Department of Pharmacology, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Esther de Boer - University Medical Center Groningen, Department of Surgery, Hanzeplein 1, 9713 GZ Groningen, Netherlands
Xian-Jin Xie - Department of Clinical Science, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Joel Thibodeaux - Department of Pathology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Rolf A Brekken - Department of Surgery, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Xiankai Sun - Department of Radiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Baran D Sumer - Department of Otolaryngology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Jinming Gao - Department of Pharmacology, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390, USA
Resource Type: Journal article
Publication Details: Nature biomedical engineering, Vol.1(1), 6
DOI: 10.1038/s41551-016-0006
PMID: 28966871
PMCID: PMC5617128
NLM abbreviation: Nat Biomed Eng
ISSN: 2157-846X
eISSN: 2157-846X
Publisher: England
Grant note: R01 EB013149 / NIBIB NIH HHS R01 CA192221 / NCI NIH HHS
Language: English
Date published: 2016
Academic Unit: Preventive and Community Dentistry; Biostatistics; Dental Research
Record Identifier: 9983917789202771

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