Journal article
A Transmembrane Accessory Subunit that Modulates Kainate-Type Glutamate Receptors
Neuron (Cambridge, Mass.), Vol.61(3), pp.385-396
02/12/2009
DOI: 10.1016/j.neuron.2008.12.014
PMCID: PMC2803770
PMID: 19217376
Abstract
Glutamate receptors play major roles in excitatory transmission in the vertebrate brain. Among ionotropic glutamate receptors (AMPA, kainate, NMDA), AMPA receptors mediate fast synaptic transmission and require TARP auxiliary subunits. NMDA receptors and kainate receptors play roles in synaptic transmission, but it remains uncertain whether these ionotropic glutamate receptors also have essential subunits. Using a proteomic screen, we have identified NETO2, a brain-specific protein of unknown function, as an interactor with kainate-type glutamate receptors. NETO2 modulates the channel properties of recombinant and native kainate receptors without affecting trafficking of the receptors and also modulates kainate-receptor-mediated mEPSCs. Furthermore, we found that kainate receptors regulate the surface expression of NETO2 and that NETO2 protein levels and surface expression are decreased in mice lacking the kainate receptor GluR6. The results show that NETO2 is a kainate receptor subunit with significant effects on glutamate signaling mechanisms in brain.
Details
- Title: Subtitle
- A Transmembrane Accessory Subunit that Modulates Kainate-Type Glutamate Receptors
- Creators
- Wei Zhang - Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520, USAFannie St-Gelais - Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USAChad P Grabner - Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USAJonathan C Trinidad - Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94143, USAAkio Sumioka - Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USAMegumi Morimoto-Tomita - Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USAKwang S Kim - Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USAChristoph Straub - Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USAAlma L Burlingame - Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94143, USAJames R Howe - Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520, USASusumu Tomita - Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA
- Resource Type
- Journal article
- Publication Details
- Neuron (Cambridge, Mass.), Vol.61(3), pp.385-396
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.neuron.2008.12.014
- PMID
- 19217376
- PMCID
- PMC2803770
- ISSN
- 0896-6273
- eISSN
- 1097-4199
- Language
- English
- Date published
- 02/12/2009
- Academic Unit
- Surgery
- Record Identifier
- 9984051771902771
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