A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ

Jacob Lam; Prasanna Katti; Michelle Biete; Margaret Mungai; Salma AshShareef; Kit Neikirk; Edgar Garza Lopez; Zer Vue; Trace A. Christensen; Heather K. Beasley; Taylor A. Rodman; Sandra A. Murray; Jeffrey L. Salisbury; Brian Glancy; Jianqiang Shao; Renata O. Pereira; E. Dale Abel; Antentor Hinton

doi:10.3390/cells10092177

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A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ

Journal article

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A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ

Jacob Lam, Prasanna Katti, Michelle Biete, Margaret Mungai, Salma AshShareef, Kit Neikirk, Edgar Garza Lopez, Zer Vue, Trace A. Christensen, Heather K. Beasley, …

Cells (Basel, Switzerland), Vol.10(9), p.2177

08/24/2021

DOI: 10.3390/cells10092177

PMCID: PMC8465115

PMID: 34571826

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Abstract

Transmission electron microscopy (TEM) is widely used as an imaging modality to provide high-resolution details of subcellular components within cells and tissues. Mitochondria and endoplasmic reticulum (ER) are organelles of particular interest to those investigating metabolic disorders. A straightforward method for quantifying and characterizing particular aspects of these organelles would be a useful tool. In this protocol, we outline how to accurately assess the morphology of these important subcellular structures using open source software ImageJ, originally developed by the National Institutes of Health (NIH). Specifically, we detail how to obtain mitochondrial length, width, area, and circularity, in addition to assessing cristae morphology and measuring mito/endoplasmic reticulum (ER) interactions. These procedures provide useful tools for quantifying and characterizing key features of sub-cellular morphology, leading to accurate and reproducible measurements and visualizations of mitochondria and ER.

Cell Biology

Life Sciences & Biomedicine

Science & Technology

Details

Title: Subtitle: A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ
Creators: Jacob Lam - University of Iowa
Prasanna Katti - National Heart Lung and Blood Institute
Michelle Biete - University of Hawaii at Hilo
Margaret Mungai - University of California, Berkeley
Salma AshShareef - University of Iowa
Kit Neikirk - University of Hawaii at Hilo
Edgar Garza Lopez - Vanderbilt University
Zer Vue - Vanderbilt University
Trace A. Christensen - Mayo Clinic
Heather K. Beasley - Vanderbilt University
Taylor A. Rodman - Vanderbilt University
Sandra A. Murray - University of Pittsburgh
Jeffrey L. Salisbury - Mayo Clinic
Brian Glancy - National Institute of Arthritis and Musculoskeletal and Skin Diseases
Jianqiang Shao - University of Iowa
Renata O. Pereira - Univ Iowa, Carver Coll Med, Dept Internal Med, 375 Newton Rd, Iowa City, IA 52242 USA
E. Dale Abel - Univ Iowa, Carver Coll Med, Dept Internal Med, 375 Newton Rd, Iowa City, IA 52242 USA
Antentor Hinton - Vanderbilt University
Resource Type: Journal article
Publication Details: Cells (Basel, Switzerland), Vol.10(9), p.2177
DOI: 10.3390/cells10092177
PMID: 34571826
PMCID: PMC8465115
NLM abbreviation: Cells
ISSN: 2073-4409
eISSN: 2073-4409
Publisher: Mdpi
Number of pages: 17
Grant note: R01HL108379; R01DK092065 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA T32 HL007121 / UNCF/BMS EE Just Grant Ford Foundation 2011577I / NSF MCB; National Science Foundation (NSF) Burroughs Wellcome Fund CASI Award; Burroughs Wellcome Fund
Language: English
Date published: 08/24/2021
Academic Unit: Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
Record Identifier: 9984420836702771

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