Journal article
A calibration curve for immobilized dihydrofolate reductase activity assay
Data in brief, Vol.4(C), pp.19-21
09/2015
DOI: 10.1016/j.dib.2015.04.005
PMCID: PMC4510376
PMID: 26217755
Abstract
An assay was developed for measuring the active-site concentration, activity, and thereby the catalytic turnover rate (
k
cat
) of an immobilized dihydrofolate reductase model system (Singh et al., (2015),
Anal. Biochem
). This data article contains a calibration plot for the developed assay. In the calibration plot rate is plotted as a function of DHFR concentration and shows linear relationship. The concentration of immobilized enzyme was varied by using 5 different size mica chips. The dsDNA concentration was the same for all chips, assuming that the surface area of the mica chip dictates the resulting amount of bound enzyme (i.e. larger sized chip would have more bound DHFR). The activity and concentration of each chip was measured.
Details
- Title: Subtitle
- A calibration curve for immobilized dihydrofolate reductase activity assay
- Creators
- Priyanka Singh - The Department of Chemistry, The University of Iowa, Iowa City, IA 52242, USAHolly Morris - The Department of Chemistry, The University of Iowa, Iowa City, IA 52242, USAAlexei V Tivanski - The Department of Chemistry, The University of Iowa, Iowa City, IA 52242, USAAmnon Kohen - The Department of Chemistry, The University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Data in brief, Vol.4(C), pp.19-21
- DOI
- 10.1016/j.dib.2015.04.005
- PMID
- 26217755
- PMCID
- PMC4510376
- NLM abbreviation
- Data Brief
- ISSN
- 2352-3409
- eISSN
- 2352-3409
- Publisher
- Elsevier
- Grant note
- DOI: 10.13039/100000002, name: NIH, award: R01GM65368; DOI: 10.13039/100000001, name: NSF, award: CHE-1149023
- Language
- English
- Date published
- 09/2015
- Academic Unit
- Chemistry
- Record Identifier
- 9984216685902771
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