Journal article
A cis-regulatory Sequence Within the yellow Locus of Drosophila melanogaster Required for Normal Male Mating Success
Genetics (Austin), Vol.172(2), pp.1009-1030
02/2006
DOI: 10.1534/genetics.105.045666
PMCID: PMC1456202
PMID: 16272418
Abstract
Drosophila melanogaster\nmales perform a courtship ritual consisting of a series of dependent fixed-action patterns. The\nyellow\n(\ny\n) gene is required for normal male courtship behavior and subsequent mating success. To better characterize the requirement for\ny\nin the manifestation of innate male sexual behavior, we measured the male mating success (MMS) of 12 hypomorphic\ny\nmutants and matched-outbred-background controls using a\ny\n+\nrescue element on a freely segregating minichromosome. We found that 4 hypomorphs significantly reduced MMS to varying degrees. Reduced MMS was largely independent of adult pigmentation patterns. These mutations defined a 300-bp regulatory region upstream of the transcription start, the mating-success regulatory sequence (MRS), whose function is required for normal MMS. Visualization of gene action via GFP and a Yellow antibody suggests that the MRS directs\ny\ntranscription in a small number of cells in the third instar CNS, the developmental stage previously implicated in the role of\ny\nwith regard to male courtship behavior. The presence of Yellow protein in these cells positively correlates with MMS in a subset of mutants. The MRS contains a regulatory sequence controlling larval pigmentation and a 35-bp sequence that is highly conserved within the genus Drosophila and is predicted to bind known transcription factors.
Details
- Title: Subtitle
- A cis-regulatory Sequence Within the yellow Locus of Drosophila melanogaster Required for Normal Male Mating Success
- Creators
- Mark David Drapeau - Department of Ecology and Evolutionary Biology, University of California, Irvine, California 92697Shawn A Cyran - Department of Ecology and Evolutionary Biology, University of California, Irvine, California 92697Michaela M Viering - Department of Ecology and Evolutionary Biology, University of California, Irvine, California 92697Pamela K Geyer - Department of Ecology and Evolutionary Biology, University of California, Irvine, California 92697Anthony D Long - Department of Ecology and Evolutionary Biology, University of California, Irvine, California 92697
- Resource Type
- Journal article
- Publication Details
- Genetics (Austin), Vol.172(2), pp.1009-1030
- DOI
- 10.1534/genetics.105.045666
- PMID
- 16272418
- PMCID
- PMC1456202
- ISSN
- 0016-6731
- eISSN
- 1943-2631
- Language
- English
- Date published
- 02/2006
- Academic Unit
- Obstetrics and Gynecology; Biochemistry and Molecular Biology
- Record Identifier
- 9984024553902771
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