A comparative study of the antigen-specific immune response induced by co-delivery of CpG ODN and antigen using fusion molecules or biodegradable microparticles

Xue-Qing Zhang; Christopher E Dahle; George J Weiner; Aliasger K Salem

doi:10.1002/jps.20978

Back

A comparative study of the antigen-specific immune response induced by co-delivery of CpG ODN and antigen using fusion molecules or biodegradable microparticles

Journal article

Peer reviewed

A comparative study of the antigen-specific immune response induced by co-delivery of CpG ODN and antigen using fusion molecules or biodegradable microparticles

Xue-Qing Zhang, Christopher E Dahle, George J Weiner and Aliasger K Salem

Journal of pharmaceutical sciences, Vol.96(12), pp.3283-3292

12/2007

DOI: 10.1002/jps.20978

PMID: 17497736

View Online

Abstract

CpG ODN are toll-like receptor 9 (TLR9) agonists that can enhance antigen presentation by antigen presenting cells (APCs) such as dendritic cells (DCs). The most potent antigen-specific responses are seen when CpG ODN and the antigen are co-localized in the same APC. CpG ODN-antigen fusion molecules and biodegradable microparticles entrapping CpG ODN and antigen can ensure both components are delivered to the same APC. In this study, we compared the efficacy of the CpG-ODN fusion molecules against biodegradable microparticles entrapping antigen and CpG ODN. Microparticles were prepared using a double emulsion solvent evaporation methodology. CpG ODN-OVA fusion molecules were prepared by mixing maleimide-activated protein with thiolated CpG ODN. Both CpG ODN-OVA fusion molecules and microparticles co-entrapping CpG ODN and OVA generated stronger IgG2a and interferon-gamma (IFN-gamma) responses than delivery of soluble CpG ODN and OVA. The microparticles generated stronger IgG2a and IFN-gamma immune responses than did CpG ODN-antigen fusion molecules.

Immunoglobulin G - blood

Ovalbumin - immunology

Dendritic Cells - immunology

Oligodeoxyribonucleotides - chemistry

Interferon-gamma - metabolism

Vaccines, Synthetic - immunology

Drug Carriers

Immunoconjugates - immunology

Alum Compounds

Adjuvants, Immunologic - chemistry

Drug Compounding

Female

Receptors, Antigen, T-Cell - immunology

Ovalbumin - chemistry

Vaccines, Synthetic - chemistry

Lactic Acid - chemistry

Oligodeoxyribonucleotides - immunology

Mice, Inbred C57BL

Cells, Cultured

Mice, Transgenic

Technology, Pharmaceutical - methods

Immunoconjugates - chemistry

Particle Size

Animals

Polyglycolic Acid - chemistry

Polymers - chemistry

Mice

Receptors, Antigen, T-Cell - genetics

Details

Title: Subtitle: A comparative study of the antigen-specific immune response induced by co-delivery of CpG ODN and antigen using fusion molecules or biodegradable microparticles
Creators: Xue-Qing Zhang - University of Iowa
Christopher E Dahle
George J Weiner
Aliasger K Salem
Resource Type: Journal article
Publication Details: Journal of pharmaceutical sciences, Vol.96(12), pp.3283-3292
DOI: 10.1002/jps.20978
PMID: 17497736
NLM abbreviation: J Pharm Sci
ISSN: 0022-3549
eISSN: 1520-6017
Publisher: United States
Grant note: P50 CA 097274 / NCI NIH HHS
Language: English
Date published: 12/2007
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Hematology, Oncology, and Blood & Marrow Transplantation; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering; Internal Medicine
Record Identifier: 9983985891602771

Metrics

28 Record Views

49 Times Cited - Web of Science

See more details