A comparison of the binding and fate of internalized neutrophil elastase in human monocytes and alveolar macrophages

S E McGowan; R D Arbeit; P J Stone; G L Snider

doi:10.1164/arrd.1983.128.4.688

Back

Journal article

A comparison of the binding and fate of internalized neutrophil elastase in human monocytes and alveolar macrophages

S E McGowan, R D Arbeit, P J Stone and G L Snider

The American review of respiratory disease, Vol.128(4), pp.688-694

10/1983

DOI: 10.1164/arrd.1983.128.4.688

PMID: 6312857

View Online

Abstract

Human alveolar macrophages (AM) bind and internalize neutrophil elastase (NE) in vitro by receptor-mediated endocytosis. Blood monocytes are progenitors of AM, and if they possess receptors for NE, they could bind and internalize NE in the pulmonary interstitium and may effect elastin degradation, which likely accompanies the development of emphysema. To determine whether monocytes contain receptors for NE, radioiodinated NE (I-NE) binding was assessed, and the results were compared with binding of I-NE to AM obtained concurrently from the same donors. The binding of I-NE to monocytes cultured in vitro for 8 days was also assessed. Specific binding of I-NE to monocytes and AM reached 80% of maximum in 30 min; similar quantities bound to AM and monocytes after a 2-h incubation with I-NE, and a smaller quantity bound to cultured monocytes. The estimated association constant for specific binding was 6 X 10(6)M-1 and 4 X 10(6)M-1 for AM and monocytes, respectively. The fate of I-NE in monocytes and AM at 24 h after uptake was assessed and compared using molecular sieve chromatography. Approximately 50% of the I-NE initially bound to either monocytes or AM remained cell-associated at the end of culture; 62 to 65% of this material eluted at 29,000 daltons and solubilized particulate elastin.

Elastin - metabolism

Pulmonary Emphysema - etiology

Neutrophils - enzymology

Humans

Receptors, Cell Surface - analysis

Cells, Cultured

Pancreatic Elastase - metabolism

Pulmonary Alveoli - enzymology

Macrophages - enzymology

Monocytes - enzymology

Endocytosis

Culture Media

Pulmonary Alveoli - metabolism

Adolescent

Adult

Binding Sites

Details

Title: Subtitle: A comparison of the binding and fate of internalized neutrophil elastase in human monocytes and alveolar macrophages
Creators: S E McGowan
R D Arbeit
P J Stone
G L Snider
Resource Type: Journal article
Publication Details: The American review of respiratory disease, Vol.128(4), pp.688-694
Publisher: United States
DOI: 10.1164/arrd.1983.128.4.688
PMID: 6312857
ISSN: 0003-0805
eISSN: 2376-3752
Grant note: 1F32-HL-06379-01 / NHLBI NIH HHS HL-25229 / NHLBI NIH HHS
Language: English
Date published: 10/1983
Academic Unit: International Programs; Internal Medicine
Record Identifier: 9983983671402771

Metrics

28 Record Views

17 Times Cited - Web of Science