A comprehensive nonredundant expressed sequence tag collection for the developing Rattus norvegicus heart

Jennifer J S Laffin; Todd E Scheetz; Maria de Fatima Bonaldo; Rebecca S Reiter; Shereen Chang; Mari Eyestone; Hakeem Abdulkawy; Bartley Brown; Chad Roberts; Dylan Tack; Tamara Kucaba; Jim Jung-Ching Lin; Val C Sheffield; Thomas L Casavant; M Bento Soares

doi:10.1152/physiolgenomics.00186.2003

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A comprehensive nonredundant expressed sequence tag collection for the developing Rattus norvegicus heart

Journal article

Peer reviewed

A comprehensive nonredundant expressed sequence tag collection for the developing Rattus norvegicus heart

Jennifer J S Laffin, Todd E Scheetz, Maria de Fatima Bonaldo, Rebecca S Reiter, Shereen Chang, Mari Eyestone, Hakeem Abdulkawy, Bartley Brown, Chad Roberts, Dylan Tack, …

Physiological genomics, Vol.17(2), pp.245-252

04/13/2004

DOI: 10.1152/physiolgenomics.00186.2003

PMID: 14762174

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Abstract

Congenital heart defects affect approximately 1,000,000 people in the United States, with 40,000 new births contributing to that number every year. A large percentage of these defects can be attributed to septal defects. We assembled a nonredundant collection of over 12,000 expressed sequence tags (ESTs) from a total of 30,000 ESTs, with the ultimate goal of identifying spatially and/or temporally regulated genes during heart septation. These ESTs were compiled from nonnormalized, normalized, and serially subtracted cDNA libraries derived from two sets of tissue samples. The first includes microdissected rat hearts from embryonic (E) days E13, E15, and E16.5-E18.5 and adult heart. The second includes hearts from embryonic days E17, E19, and E21 and postnatal (P) days P1, P12, P74, and P200. Over 6,000 novel ESTs were identified in the libraries derived from these two sets of tissues, all of which have been contributed to the NCBI rat UniGene collection. It is anticipated that such EST and cDNA clone resources will prove invaluable to gene expression studies aimed at the understanding of the molecular mechanisms underlying heart septation defects.

Animals

Gene Library

Rats - embryology

Heart - embryology

Heart - growth & development

Myocardium - metabolism

Rats - genetics

Rats, Sprague-Dawley

Rats - growth & development

Expressed Sequence Tags

Details

Title: Subtitle: A comprehensive nonredundant expressed sequence tag collection for the developing Rattus norvegicus heart
Creators: Jennifer J S Laffin - Department of Pediatrics and Interdepartmental-Genetics Graduate Program, The University of Iowa, Iowa City, Iowa 52242, USA. jennifer-laffin@uiowa.edu
Todd E Scheetz
Maria de Fatima Bonaldo
Rebecca S Reiter
Shereen Chang - University of Iowa
Mari Eyestone
Hakeem Abdulkawy
Bartley Brown
Chad Roberts
Dylan Tack
Tamara Kucaba
Jim Jung-Ching Lin
Val C Sheffield
Thomas L Casavant
M Bento Soares
Resource Type: Journal article
Publication Details: Physiological genomics, Vol.17(2), pp.245-252
DOI: 10.1152/physiolgenomics.00186.2003
PMID: 14762174
NLM abbreviation: Physiol Genomics
ISSN: 1531-2267
eISSN: 1531-2267
Publisher: United States
Grant note: 2R01-HL-59789 / NHLBI NIH HHS
Language: English
Date published: 04/13/2004
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Medical Genetics and Genomics; Biology; Ophthalmology and Visual Sciences
Record Identifier: 9983980067702771

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