A comprehensive promoter landscape identifies a novel promoter for CD133 in restricted tissues, cancers, and stem cells

Ramakrishna Sompallae; Oliver Hofmann; Christopher A. Maher; Craig Gedye; Andreas Behren; Morana Vitezic; Carsten O. Daub; Sylvie Devalle; Otavia L. Caballero; Piero Carninci; Yoshihide Hayashizaki; Elizabeth R. Lawlor; Jonathan Cebon; Winston Hide

doi:10.3389/fgene.2013.00209

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A comprehensive promoter landscape identifies a novel promoter for CD133 in restricted tissues, cancers, and stem cells

Journal article

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A comprehensive promoter landscape identifies a novel promoter for CD133 in restricted tissues, cancers, and stem cells

Ramakrishna Sompallae, Oliver Hofmann, Christopher A. Maher, Craig Gedye, Andreas Behren, Morana Vitezic, Carsten O. Daub, Sylvie Devalle, Otavia L. Caballero, Piero Carninci, …

Frontiers in genetics, Vol.4, pp.209-209

2013

DOI: 10.3389/fgene.2013.00209

PMCID: PMC3810939

PMID: 24194746

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Abstract

PROM1 is the gene encoding prominin-1 or CD133, an important cell surface marker for the isolation of both normal and cancer stem cells. PROM1 transcripts initiate at a range of transcription start sites (TSS) associated with distinct tissue and cancer expression profiles. Using high resolution Cap Analysis of Gene Expression (CAGE) sequencing we characterize TSS utilization across a broad range of normal and developmental tissues. We identify a novel proximal promoter (P6) within CD133 + melanoma cell lines and stem cells. Additional exon array sampling finds P6 to be active in populations enriched for mesenchyme, neural stem cells and within CD133 + enriched Ewing sarcomas. The P6 promoter is enriched with respect to previously characterized PROM1 promoters for a HMGI/Y (HMGA1) family transcription factor binding site motif and exhibits different epigenetic modifications relative to the canonical promoter region of PROM1.

Oncology

Details

Title: Subtitle: A comprehensive promoter landscape identifies a novel promoter for CD133 in restricted tissues, cancers, and stem cells
Creators: Ramakrishna Sompallae - Harvard University
Oliver Hofmann - Harvard University
Christopher A. Maher - Washington University in St. Louis
Craig Gedye - Ludwig Cancer Research
Andreas Behren - Ludwig Cancer Research
Morana Vitezic - Karolinska Institutet
Carsten O. Daub - Karolinska Institutet
Sylvie Devalle - Memorial Sloan Kettering Cancer Center
Otavia L. Caballero - Johns Hopkins University
Piero Carninci - RIKEN Center for Integrative Medical Sciences
Yoshihide Hayashizaki - RIKEN Center for Integrative Medical Sciences
Elizabeth R. Lawlor - University of Michigan–Ann Arbor
Jonathan Cebon - Ludwig Cancer Research
Winston Hide - Harvard Stem Cell Institute
Resource Type: Journal article
Publication Details: Frontiers in genetics, Vol.4, pp.209-209
Publisher: Frontiers Media S.A
DOI: 10.3389/fgene.2013.00209
PMID: 24194746
PMCID: PMC3810939
ISSN: 1664-8021
eISSN: 1664-8021
Language: English
Date published: 2013
Academic Unit: Pathology
Record Identifier: 9984622057502771

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