Journal article
A critical component of meiotic drive in Neurospora is located near a chromosome rearrangement
Genetics (Austin), Vol.197(4), pp.1165-1174
08/2014
DOI: 10.1534/genetics.114.167007
PMCID: PMC4125391
PMID: 24931406
Abstract
Neurospora fungi harbor a group of meiotic drive elements known as Spore killers (Sk). Spore killer-2 (Sk-2) and Spore killer-3 (Sk-3) are two Sk elements that map to a region of suppressed recombination. Although this recombination block is limited to crosses between Sk and Sk-sensitive (Sk(S)) strains, its existence has hindered Sk characterization. Here we report the circumvention of this obstacle by combining a classical genetic screen with next-generation sequencing technology and three-point crossing assays. This approach has allowed us to identify a novel locus called rfk-1, mutation of which disrupts spore killing by Sk-2. We have mapped rfk-1 to a 45-kb region near the right border of the Sk-2 element, a location that also harbors an 11-kb insertion (Sk-2(INS1)) and part of a >220-kb inversion (Sk-2(INV1)). These are the first two chromosome rearrangements to be formally identified in a Neurospora Sk element, providing evidence that they are at least partially responsible for Sk-based recombination suppression. Additionally, the proximity of these chromosome rearrangements to rfk-1 (a critical component of the spore-killing mechanism) suggests that they have played a key role in the evolution of meiotic drive in Neurospora.
Details
- Title: Subtitle
- A critical component of meiotic drive in Neurospora is located near a chromosome rearrangement
- Creators
- Austin M Harvey - School of Biological Sciences, Illinois State University, Normal, Illinois 61790David G Rehard - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211Katie M Groskreutz - School of Biological Sciences, Illinois State University, Normal, Illinois 61790Danielle R Kuntz - School of Biological Sciences, Illinois State University, Normal, Illinois 61790Kevin J Sharp - School of Biological Sciences, Illinois State University, Normal, Illinois 61790Patrick K T Shiu - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211Thomas M Hammond - School of Biological Sciences, Illinois State University, Normal, Illinois 61790 tmhammo@ilstu.edu
- Resource Type
- Journal article
- Publication Details
- Genetics (Austin), Vol.197(4), pp.1165-1174
- DOI
- 10.1534/genetics.114.167007
- PMID
- 24931406
- PMCID
- PMC4125391
- ISSN
- 0016-6731
- eISSN
- 1943-2631
- Grant note
- P01 GM068087 / NIGMS NIH HHS
- Language
- English
- Date published
- 08/2014
- Academic Unit
- Biology
- Record Identifier
- 9984083283002771
Metrics
29 Record Views