A cytoplasmic coiled-coil domain is required for histidine kinase activity of the yeast osmosensor, SLN1

Wei Tao; Cheryl L Malone; Addison D Ault; Robert J Deschenes; Jan S Fassler

doi:10.1046/j.1365-2958.2002.02757.x

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A cytoplasmic coiled-coil domain is required for histidine kinase activity of the yeast osmosensor, SLN1

Journal article

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Peer reviewed

A cytoplasmic coiled-coil domain is required for histidine kinase activity of the yeast osmosensor, SLN1

Wei Tao, Cheryl L Malone, Addison D Ault, Robert J Deschenes and Jan S Fassler

Molecular microbiology, Vol.43(2), pp.459-473

01/2002

DOI: 10.1046/j.1365-2958.2002.02757.x

PMCID: PMC2892222

PMID: 11985722

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Abstract

The yeast histidine kinase, Sln1p, is a plasma membrane-associated osmosensor that regulates the activity of the osmotic stress MAP kinase pathway. Changes in the osmotic environment of the cell influence the autokinase activity of the cytoplasmic kinase domain of Sln1p. Neither the nature of the stimulus, the mechanism by which the osmotic signal is transduced nor the manner in which the kinase is regulated is currently clear. We have identified several mutations located in the linker region of the Sln1 kinase (just upstream of the kinase domain) that cause hyperactivity of the Sln1 kinase. This region of histidine kinases is largely uncharacterized, but its location between the transmembrane domains and the cytoplasmic kinase domain suggests that it may have a potential role in signal transduction. In this study, we have investigated the Sln1 linker region in order to understand its function in signal transduction and regulation of Sln1 kinase activity. Our results indicate that the linker region forms a coiled-coil structure and suggest a mechanism by which alterations induced by osmotic stress influence kinase activity by altering the alignment of the phospho-accepting histidine with respect to the catalytic domain of the kinase.

Amino Acid Sequence

Binding Sites

CCAAT-Enhancer-Binding Proteins - metabolism

Cytoplasm

Fungal Proteins - genetics

Fungal Proteins - metabolism

Intracellular Signaling Peptides and Proteins

Leucine Zippers

Molecular Sequence Data

Mutagenesis

Phenotype

Protein Kinases - genetics

Protein Kinases - metabolism

Protein Structure, Tertiary

Recombinant Fusion Proteins - genetics

Recombinant Fusion Proteins - metabolism

Saccharomyces cerevisiae - enzymology

Saccharomyces cerevisiae - genetics

Saccharomyces cerevisiae - growth & development

Saccharomyces cerevisiae Proteins

Structure-Activity Relationship

Details

Title: Subtitle: A cytoplasmic coiled-coil domain is required for histidine kinase activity of the yeast osmosensor, SLN1
Creators: Wei Tao - Department of Biological Sciences, University of Iowa, Iowa City 52242, USA
Cheryl L Malone
Addison D Ault
Robert J Deschenes
Jan S Fassler
Resource Type: Journal article
Publication Details: Molecular microbiology, Vol.43(2), pp.459-473
DOI: 10.1046/j.1365-2958.2002.02757.x
PMID: 11985722
PMCID: PMC2892222
ISSN: 0950-382X
eISSN: 1365-2958
Grant note: R01 GM068746 / NIGMS NIH HHS T32AG / NIA NIH HHS R01 GM068746-05 / NIGMS NIH HHS R01 GM056719 / NIGMS NIH HHS R01 GM056719-05 / NIGMS NIH HHS GM56719 / NIGMS NIH HHS
Language: English
Date published: 01/2002
Academic Unit: Biology
Record Identifier: 9984217417402771

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