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A deficiency in the B cell response of C57BL/6 mice correlates with loss of macrophage-mediated killing of Leishmania amazonensis
Journal article   Peer reviewed

A deficiency in the B cell response of C57BL/6 mice correlates with loss of macrophage-mediated killing of Leishmania amazonensis

Katherine N Gibson-Corley, Paola M Boggiatto, Rami M Mukbel, Christine A Petersen and Douglas E Jones
International journal for parasitology, Vol.40(2), pp.157-161
02/2010
DOI: 10.1016/j.ijpara.2009.11.010
PMCID: PMC2814795
PMID: 20004204
url
https://www.ncbi.nlm.nih.gov/pmc/articles/2814795View
Open Access

Abstract

Infection of C3HeB/FeJ and C57BL/6 mice with Leishmania major stimulates a healing cell-mediated immune response, while Leishmania amazonensis infection leads to chronic disease. Here we show C3HeB/FeJ mice co-infected with both species of Leishmania heal, while co-infected C57BL/6 mice do not. Using an in vitro killing assay we determined B cells from infected C57BL/6 mice are ineffective in promoting parasite killing compared with B cells from infected C3HeB/FeJ mice. Furthermore, infected C57BL/6 mice produce less antigen-specific antibodies compared with infected C3HeB/FeJ mice. These findings suggest B cells play a required role in the cell-mediated immune response against L. amazonensis.
Cutaneous leishmaniasis Co-infection B cells Macrophages Murine model

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