A deficit of functional GABA(A) receptors in neurons of beta 3 subunit knockout mice

Matthew D Krasowski; Caroline E Rick; Neil L Harrison; Leonard L Firestone; Gregg E Homanics

doi:10.1016/s0304-3940(97)00929-4

Back

A deficit of functional GABA(A) receptors in neurons of beta 3 subunit knockout mice

Journal article

Open access

Peer reviewed

A deficit of functional GABA(A) receptors in neurons of beta 3 subunit knockout mice

Matthew D Krasowski, Caroline E Rick, Neil L Harrison, Leonard L Firestone and Gregg E Homanics

Neuroscience letters, Vol.240(2), pp.81-84

01/09/1998

DOI: 10.1016/s0304-3940(97)00929-4

PMID: 9486477

Files and links (1)

url

https://www.ncbi.nlm.nih.gov/pmc/articles/2846962View

Open Access

Abstract

Mice whose gamma-aminobutyric acid type A (GABA(A)) beta3 subunit gene is inactivated ('beta3 knockout mice') have been previously shown to have epilepsy, hypersensitive behavior, cleft palate, and a high incidence of neonatal mortality. In this study, we analyze whole-cell responses to GABA in neurons from beta3+/+, beta3+/- and beta3-/- mice. We demonstrate markedly decreased responses to GABA in both hippocampal and dorsal root ganglion neurons isolated from beta3-/- mice without major differences in the GABA concentration-response curves. We also utilize the subunit selective pharmacology of Zn2+ and the anticonvulsant drug loreclezole to help infer the presence of beta2 and gamma subunits in the GABA(A) receptors remaining in neurons from beta3-/- mice.

Cells, Cultured

Ganglia, Spinal - physiology

Neurons - cytology

Hippocampus - drug effects

Receptors, GABA-A - deficiency

Drug Synergism

Hippocampus - metabolism

Patch-Clamp Techniques

Animals

Receptors, GABA-A - genetics

Neurons - physiology

Receptors, GABA-A - drug effects

Mice

Neurons - metabolism

Mice, Knockout - physiology

Ganglia, Spinal - drug effects

Hippocampus - physiology

Ganglia, Spinal - metabolism

Details

Title: Subtitle: A deficit of functional GABA(A) receptors in neurons of beta 3 subunit knockout mice
Creators: Matthew D Krasowski - Committee on Neurobiology, University of Chicago, IL 60637, USA
Caroline E Rick
Neil L Harrison
Leonard L Firestone
Gregg E Homanics
Resource Type: Journal article
Publication Details: Neuroscience letters, Vol.240(2), pp.81-84
DOI: 10.1016/s0304-3940(97)00929-4
PMID: 9486477
NLM abbreviation: Neurosci Lett
ISSN: 0304-3940
eISSN: 1872-7972
Publisher: Ireland
Grant note: F30 MH011504 / NIMH NIH HHS F30 MH011504-02 / NIMH NIH HHS F30 MH011504-01 / NIMH NIH HHS
Language: English
Date published: 01/09/1998
Academic Unit: Pathology
Record Identifier: 9984047711602771

Metrics

34 Record Views

38 Times Cited - Web of Science