A delayed proinflammatory response of human preadipocytes to PCB126 is dependent on the aryl hydrocarbon receptor

Francoise A Gourronc; Larry W Robertson; Aloysius J Klingelhutz

doi:10.1007/s11356-017-9676-z

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A delayed proinflammatory response of human preadipocytes to PCB126 is dependent on the aryl hydrocarbon receptor

Journal article

Peer reviewed

A delayed proinflammatory response of human preadipocytes to PCB126 is dependent on the aryl hydrocarbon receptor

Francoise A Gourronc, Larry W Robertson and Aloysius J Klingelhutz

Environmental science and pollution research international, Vol.25(17), pp.16481-16492

06/2018

DOI: 10.1007/s11356-017-9676-z

PMCID: PMC5764822

PMID: 28699004

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Abstract

Inflammation in adipose tissue is recognized as a causative factor in the development of type II diabetes. Adipocyte hypertrophy as well as bacterial and environmental factors have been implicated in causing inflammation in mature adipocytes. Exposure to persistent organic pollutants such as polychlorinated biphenyls (PCBs) has been associated with the development of type II diabetes. We show here that PCB126, a dioxin-like PCB, activates a robust proinflammatory state in fat cell precursors (preadipocytes). The response was found to be dependent on aryl hydrocarbon receptor (AhR) activation, although induction of the response was delayed compared to upregulation of CYP1A1, a classic AhR-responsive gene. Treatment of preadipocytes with a nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) inhibitor partially attenuated the PCB126-induced inflammatory response and partly, but not completely, ameliorated disruption of adipogenesis caused by PCB126. Our results indicate a role for PCB126 in mediating an inflammatory response through AhR in preadipocytes that interferes with adipogenesis.

Inflammation - chemically induced

Environmental Pollutants - adverse effects

Polychlorinated Biphenyls - pharmacology

Receptors, Aryl Hydrocarbon - chemistry

Humans

NF-kappa B - chemistry

Diabetes Mellitus, Type 2 - metabolism

Dioxins - chemistry

Polychlorinated Biphenyls - chemistry

Environmental Pollutants - chemistry

Polychlorinated Dibenzodioxins - chemistry

Receptors, Aryl Hydrocarbon - metabolism

Adipocytes - chemistry

ISRP Project 1 2015-2020

Synthesis Core

Details

Title: Subtitle: A delayed proinflammatory response of human preadipocytes to PCB126 is dependent on the aryl hydrocarbon receptor
Creators: Francoise A Gourronc - Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, 52242, USA
Larry W Robertson - Department of Occupational and Environmental Health, College of Public Health, University of Iowa, Iowa City, IA, 52242, USA
Aloysius J Klingelhutz - Department of Microbiology and Immunology, Carver College of Medicine, The University of Iowa, 2202 MERF, 375 Newton Road, Iowa City, IA, 52242, USA. al-klingelhutz@uiowa.edu
Resource Type: Journal article
Publication Details: Environmental science and pollution research international, Vol.25(17), pp.16481-16492
DOI: 10.1007/s11356-017-9676-z
PMID: 28699004
PMCID: PMC5764822
NLM abbreviation: Environ Sci Pollut Res Int
ISSN: 0944-1344
eISSN: 1614-7499
Publisher: Germany
Grant note: P30 ES005605 / NIEHS NIH HHS U54 TR001356 / NCATS NIH HHS P42 ES013661 / National Institute of Environmental Health Sciences P42 ES013661 / NIEHS NIH HHS P30 CA086862 / NCI NIH HHS P30 ES05605 / National Institute of Environmental Health Sciences
Language: English
Date published: 06/2018
Academic Unit: Microbiology and Immunology; Occupational and Environmental Health; Radiation Oncology; Iowa Superfund Research Program
Record Identifier: 9984001214102771

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