A differentially expressed gene family encoding amastin, a surface protein of Trypanosoma cruzi amastigotes

Santuza M R Teixeira; David G Russell; Louis V Kirchhoff; John E Donelson

doi:10.1016/S0021-9258(17)32022-7

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A differentially expressed gene family encoding amastin, a surface protein of Trypanosoma cruzi amastigotes

Journal article

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A differentially expressed gene family encoding amastin, a surface protein of Trypanosoma cruzi amastigotes

Santuza M R Teixeira, David G Russell, Louis V Kirchhoff and John E Donelson

The Journal of biological chemistry, Vol.269(32), pp.20509-20516

1994

DOI: 10.1016/S0021-9258(17)32022-7

PMID: 8051148

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Abstract

A new family of closely related glycoproteins, collectively called amastins, has been found on the surface of the amastigote form of Trypanosoma cruzi. The gene family encoding these amastigote-specific proteins was identified by differentially screening an amastigote cDNA library with reverse transcribed poly(A)+ RNA from amastigote and epimastigote stages of the parasite. Amastins are encoded by eight or more tandem genes, at least five of which are distinguished by nucleotide point changes. The 1.4-kilobase amastin mRNAs are 50 times more abundant in amastigotes than in epimastigotes or trypomastigotes. The amastin genes are transcribed to an equal extent in both amastigotes and epimastigotes, indicating that the stage-specific amastin mRNA levels are determined by a post-transcriptional mechanism. Sequence determination of full-length cDNAs reveals an open reading frame encoding 174 amino acids and a 700-base pair 3'-untranslated region. Nascent amastins contain four distinct hydrophobic regions of 20-30 amino acids each, 2 at internal locations and 1 each at the N and C termini.

Analytical, structural and metabolic biochemistry

Biochemistry. Physiology. Immunology. Molecular biology

Biological and medical sciences

Fundamental and applied biological sciences. Psychology

Glycoproteins

Proteins

Protozoa

Details

Title: Subtitle: A differentially expressed gene family encoding amastin, a surface protein of Trypanosoma cruzi amastigotes
Creators: Santuza M R Teixeira - University of Iowa
David G Russell - Washington University in St. Louis
Louis V Kirchhoff - University of Iowa
John E Donelson - University of Iowa
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.269(32), pp.20509-20516
DOI: 10.1016/S0021-9258(17)32022-7
PMID: 8051148
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: American Society for Biochemistry and Molecular Biology
Language: English
Date published: 1994
Academic Unit: Psychiatry; Infectious Diseases; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9984280866402771

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