Journal article
A direct comparison of mouse and human intestinal development using epithelial gene expression patterns
Pediatric research, Vol.88(1), pp.66-76
07/01/2020
DOI: 10.1038/s41390-019-0472-y
PMCID: PMC6930976
PMID: 31242501
Abstract
Background Preterm infants are susceptible to unique pathology due to their immaturity. Mouse models are commonly used to study immature intestinal disease, including necrotizing enterocolitis (NEC). Current NEC models are performed at a variety of ages, but data directly comparing intestinal developmental stage equivalency between mice and humans are lacking. Methods Small intestines were harvested from C57BL/6 mice at 3-4 days intervals from birth to P28 (n = 8 at each age). Preterm human small intestine samples representing 17-23 weeks of completed gestation were obtained from the University of Pittsburgh Health Sciences Tissue Bank, and at term gestation during reanastamoses after resection for NEC (n = 4-7 at each age). Quantification of intestinal epithelial cell types and messenger RNA for marker genes were evaluated on both species. Results Overall, murine and human developmental trends over time are markedly similar. Murine intestine prior to P10 is most similar to human fetal intestine prior to viability. Murine intestine at P14 is most similar to human intestine at 22-23 weeks completed gestation, and P28 murine intestine is most similar to human term intestine. Conclusion Use of C57BL/6J mice to model the human immature intestine is reasonable, but the age of mouse chosen is a critical factor in model development.
Details
- Title: Subtitle
- A direct comparison of mouse and human intestinal development using epithelial gene expression patterns
- Creators
- Amy H. Stanford - University of IowaHuiyu Gong - University of IowaMackenzie Noonan - University of IowaAngela N. Lewis - Washington University in St. LouisQingqing Gong - Washington University in St. LouisWyatt E. Lanik - Washington University in St. LouisJonathan J. Hsieh - Children's Hospital of Los AngelesShiloh R. Lueschow - University of IowaMark R. Frey - Children's Hospital of Los AngelesMisty Good - Washington University in St. Louis School of MedicineSteven J. McElroy - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Pediatric research, Vol.88(1), pp.66-76
- DOI
- 10.1038/s41390-019-0472-y
- PMID
- 31242501
- PMCID
- PMC6930976
- NLM abbreviation
- Pediatr Res
- ISSN
- 0031-3998
- eISSN
- 1530-0447
- Publisher
- Springer Nature
- Number of pages
- 11
- Grant note
- R01DK095004 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) R03 DK097335; R01 DK118568; R01 DK095004; R03 DK111473; K08 DK101608 / NIDDK NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
- Language
- English
- Date published
- 07/01/2020
- Academic Unit
- Stead Family Department of Pediatrics; Neonatology; Internal Medicine
- Record Identifier
- 9984354009102771
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