A direct interaction between a glutamine-rich activator and the N terminus of TFIIB can mediate transcriptional activation in vivo

John Colgan; Harry Ashali; James L Manley

doi:10.1128/MCB.15.4.2311

Back

A direct interaction between a glutamine-rich activator and the N terminus of TFIIB can mediate transcriptional activation in vivo

Journal article

Peer reviewed

A direct interaction between a glutamine-rich activator and the N terminus of TFIIB can mediate transcriptional activation in vivo

John Colgan, Harry Ashali and James L Manley

Molecular and cellular biology, Vol.15(4), pp.2311-2320

04/1995

DOI: 10.1128/MCB.15.4.2311

PMCID: PMC230459

PMID: 7891725

View Online

Abstract

Studies examining the mechanism by which transcriptional activators function have suggested that the general transcription factor IIB (TFIIB) can be a target for certain regulatory proteins. For example, we showed previously that expression of a mutant form of TFIIB can specifically inhibit activation in vivo mediated by the strong, glutamine-rich activator protein GAL4-ftzQ. Using transient cotransfection assays, we have defined the regions in both GAL4-ftzQ and TFIIB that are required for activity in vivo and provide evidence that a potential zinc finger structure at the N terminus of TFIIB is necessary for the observed functional interaction between the two proteins. Using a protein binding assay, we have demonstrated that GAL4-ftzQ can specifically interact with TFIIB in vitro. This interaction requires the same regions in both molecules necessary for function in vivo and is reduced or eliminated by mutations predicted to disrupt the zinc finger in TFIIB. These results support the idea that a direct interaction between a regulatory protein and TFIIB can be important for transcriptional activation in vivo and, combined with previous data of others, suggest that different activators can function by contacting distinct regions of TFIIB.

Amino Acid Sequence

Drosophila Proteins

Zinc Fingers

Cells, Cultured

Molecular Sequence Data

Drosophila - cytology

Structure-Activity Relationship

RNA Polymerase II - metabolism

Fushi Tarazu Transcription Factors

Recombinant Fusion Proteins

Transcription Factors - metabolism

Animals

Transcription Factor TFIIB

DNA Mutational Analysis

Protein Binding

Trans-Activators - metabolism

Transcription, Genetic

Details

Title: Subtitle: A direct interaction between a glutamine-rich activator and the N terminus of TFIIB can mediate transcriptional activation in vivo
Creators: John Colgan - Department of Biological Sciences, Columbia University, New York, New York 10027
Harry Ashali
James L Manley
Resource Type: Journal article
Publication Details: Molecular and cellular biology, Vol.15(4), pp.2311-2320
DOI: 10.1128/MCB.15.4.2311
PMID: 7891725
PMCID: PMC230459
NLM abbreviation: Mol Cell Biol
ISSN: 0270-7306
eISSN: 1098-5549
Publisher: United States
Grant note: GM 37971 / NIGMS NIH HHS
Language: English
Date published: 04/1995
Academic Unit: Anatomy and Cell Biology; Immunology; Internal Medicine
Record Identifier: 9984025327402771

Metrics

33 Record Views

40 Times Cited - Web of Science