Journal article
A distinguishing profile of chemokines, cytokines and biomarkers in the saliva of children with Sjögren’s syndrome
Rheumatology (Oxford, England), Vol.60(10), pp.4765-4777
01/29/2021
DOI: 10.1093/rheumatology/keab098
PMID: 33512494
Abstract
Abstract Objective SS is an autoimmune disease most commonly diagnosed in adults but can occur in children. Our objective was to assess the presence of chemokines, cytokines and biomarkers (CCBMs) in saliva from these children that were associated with lymphocyte and mononuclear cell functions. Methods Saliva was collected from 11 children diagnosed with SS prior to age 18 years and 16 normal healthy children. A total of 105 CCBMs were detected in multiplex microparticle-based immunoassays. ANOVA and t test (0.05 level) were used to detect differences. Ingenuity Pathway Analysis (IPA) was used to assess whether elevated CCBMs were in annotations associated with immune system diseases and select leukocyte activities and functions. Machine learning methods were used to evaluate the predictive power of these CCBMs for SS and were measured by receiver operating characteristic (ROC) curve and area under curve (AUC). Results Of the 105 CCBMs detected, 43 (40.9%) differed in children with SS from those in healthy study controls (P < 0.05) and could differentiate the two groups (P < 0.05). Elevated CCBMs in IPA annotations were associated with autoimmune diseases and with leukocyte chemotaxis, migration, proliferation, and regulation of T cell activation. The best AUC value in ROC analysis was 0.93, indicating that there are small numbers of CCBMs that may be useful for diagnosis of SS. Conclusion While 35 of these 43 CCBMs have been previously reported in SS, 8 CCBMs had not. Additional studies focusing on these CCBMs may provide further insight into disease pathogenesis and may contribute to diagnosis of SS in children.
Details
- Title: Subtitle
- A distinguishing profile of chemokines, cytokines and biomarkers in the saliva of children with Sjögren’s syndrome
- Creators
- M Paula Gomez Hernandez - Pediatric DentistryEmily E Starman - Iowa Institute for Oral Health Research, College of DentistryAndrew B Davis - Department of Otolaryngology, College of MedicineMiyuraj Harishchandra Hikkaduwa Withanage - Division of Biostatistics and Computational Biology, College of DentistryErliang Zeng - Division of Biostatistics and Computational Biology, College of DentistryScott M Lieberman - Division of Rheumatology, Allergy and Immunology, Stead Family Department of Pediatrics, Carver College of MedicineKim A Brogden - Iowa Institute for Oral Health Research, College of DentistryEmily A Lanzel - Department of Oral Pathology, Radiology and Medicine, College of Dentistry, University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Rheumatology (Oxford, England), Vol.60(10), pp.4765-4777
- DOI
- 10.1093/rheumatology/keab098
- PMID
- 33512494
- NLM abbreviation
- Rheumatology (Oxford)
- ISSN
- 1462-0324
- eISSN
- 1462-0332
- Grant note
- name: Pilot Research Grant from the Sjögren’s Syndrome Foundation
- Language
- English
- Date published
- 01/29/2021
- Academic Unit
- Preventive and Community Dentistry; Roy J. Carver Department of Biomedical Engineering; Oral Pathology, Radiology and Medicine; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Biostatistics; Rheumatology, Allergy, and Immunology; Dental Research; Periodontics
- Record Identifier
- 9984071934902771
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