Journal article
A dual role for Cav1.4 Ca2+ channels in the molecular and structural organization of the rod photoreceptor synapse
eLife, Vol.9, e62184
09/17/2020
DOI: 10.7554/eLife.62184
PMCID: PMC7561352
PMID: 32940604
Abstract
Synapses are fundamental information processing units that rely on voltage-gated Ca2+ (Cav) channels to trigger Ca2+-dependent neurotransmitter release. Cav channels also play Ca2+-independent roles in other biological contexts, but whether they do so in axon terminals is unknown. Here, we addressed this unknown with respect to the requirement for Cav1.4 L-type channels for the formation of rod photoreceptor synapses in the retina. Using a mouse strain expressing a non-conducting mutant form of Cav1.4, we report that the Cav1.4 protein, but not its Ca2+ conductance, is required for the molecular assembly of rod synapses; however, Cav1.4 Ca2+ signals are needed for the appropriate recruitment of postsynaptic partners. Our results support a model in which presynaptic Cav channels serve both as organizers of synaptic building blocks and as sources of Ca2+ ions in building the first synapse of the visual pathway and perhaps more broadly in the nervous system.
Details
- Title: Subtitle
- A dual role for Cav1.4 Ca2+ channels in the molecular and structural organization of the rod photoreceptor synapse
- Creators
- J Wesley Maddox - Molecular Physiology and Biophysics, University of Iowa, Iowa City, United StatesKate L Randall - Molecular Physiology and Biophysics, University of Iowa, Iowa City, United StatesRavi P Yadav - Molecular Physiology and Biophysics, University of Iowa, Iowa City, United StatesBrittany Williams - Molecular Physiology and Biophysics, University of Iowa, Iowa City, United StatesJussara Hagen - Molecular Physiology and Biophysics, University of Iowa, Iowa City, United StatesPaul J Derr - Neuroscience, University of Wisconsin, Madison, Madison, United StatesVasily Kerov - Molecular Physiology and Biophysics, University of Iowa, Iowa City, United StatesLuca Della Santina - Department of Ophthalmology, University of California, San Francisco, San Francisco, United StatesSheila A Baker - Biochemistry, University of Iowa, Iowa City, United StatesNikolai Artemyev - Molecular Physiology, University of Iowa Carver College of Medicine, Iowa City, United StatesMrinalini Hoon - Department of Neuroscience, University of Wisconsin, Madison, Madison, United StatesAmy Lee - Molecular Physiology and Biophysics, University of Iowa, Iowa City, United States
- Resource Type
- Journal article
- Publication Details
- eLife, Vol.9, e62184
- DOI
- 10.7554/eLife.62184
- PMID
- 32940604
- PMCID
- PMC7561352
- NLM abbreviation
- Elife
- ISSN
- 2050-084X
- eISSN
- 2050-084X
- Grant note
- DOI: 10.13039/100000053, name: National Eye Institute, award: EY 026817; name: McPherson Eye Research Institute; DOI: 10.13039/100001818, name: Research to Prevent Blindness; DOI: 10.13039/100000053, name: National Eye Institute, award: EY 029953; DOI: 10.13039/100000053, name: National Eye Institute, award: EY 026477; DOI: 10.13039/100000053, name: National Eye Institute, award: EY010843; DOI: 10.13039/100000053, name: National Eye Institute, award: EY012682
- Language
- English
- Date published
- 09/17/2020
- Academic Unit
- Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Biochemistry and Molecular Biology; Ophthalmology and Visual Sciences
- Record Identifier
- 9984070263102771
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