Journal article
A functional AMPA receptor–calcium channel complex in the postsynaptic membrane
Proceedings of the National Academy of Sciences - PNAS, Vol.103(14), pp.5561-5566
04/04/2006
DOI: 10.1073/pnas.0601289103
PMCID: PMC1459393
PMID: 16567654
Abstract
Ca2+ channels play critical roles in the regulation of synaptic activity. In contrast to the well established function of voltage-activated Ca2+ channels in the presynaptic membrane for neurotransmitter release, some studies are just beginning to elucidate the functions of the Ca2+ channels in the postsynaptic membrane. In this study, we demonstrated the functional association of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors with the neuronal Ca2+ channels. A series of biochemical studies showed the specific association of Cav2.1 (α1A-class) and Cav2.2 (α1B-class) with AMPA receptors in the postsynaptic membrane. Our electrophysiological and Ca2+ imaging analyses of recombinant Cav2.1 and AMPA receptors also showed functional coupling of the two channels. Considering the critical roles of postsynaptic intracellular concentration of Ca2+ ([Ca2+]i) increase and AMPA receptor trafficking for long-term potentiation (LTP) and long-term depression (LTD), the functional association of Ca2+ channels with the AMPA receptors may provide new insights into the mechanism of synaptic plasticity.
Details
- Title: Subtitle
- A functional AMPA receptor–calcium channel complex in the postsynaptic membrane
- Creators
- Myoung-Goo Kang - Howard Hughes Medical Institute and Departments of Physiology and Biophysics, Internal Medicine, and Neurology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242; andChien-Chang Chen - Howard Hughes Medical Institute and Departments of Physiology and Biophysics, Internal Medicine, and Neurology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242; andMinoru Wakamori - Laboratory of Molecular Biology, Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, JapanYuji Hara - Laboratory of Molecular Biology, Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, JapanYasuo Mori - Laboratory of Molecular Biology, Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, JapanKevin P Campbell - Howard Hughes Medical Institute and Departments of Physiology and Biophysics, Internal Medicine, and Neurology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242; and
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.103(14), pp.5561-5566
- DOI
- 10.1073/pnas.0601289103
- PMID
- 16567654
- PMCID
- PMC1459393
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 04/04/2006
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984020876402771
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