Journal article
A general life-death selection strategy for dissecting protein functions
Nature methods, Vol.6(11), pp.813-U45
11/01/2009
DOI: 10.1038/NMETH.1389
PMID: 19820714
Abstract
Clonal selection strategies are central tools in molecular biology. We developed a general strategy to dissect protein functions through positive and negative clonal selection for protein-protein interactions, based on a protein-fragment complementation assay using Saccharomyces cerevisiae cytosine deaminase as a reporter. We applied this method to mutational or chemical disruption of protein-protein interactions in yeast and to dissection of the functions of an allosterically activated transcription factor, Swi6.
Details
- Title: Subtitle
- A general life-death selection strategy for dissecting protein functions
- Creators
- Po Hien Ear - Université de MontréalStephen W. Michnick - Université de Montréal
- Resource Type
- Journal article
- Publication Details
- Nature methods, Vol.6(11), pp.813-U45
- Publisher
- Springer Nature
- DOI
- 10.1038/NMETH.1389
- PMID
- 19820714
- ISSN
- 1548-7091
- eISSN
- 1548-7105
- Number of pages
- 7
- Grant note
- Faculte des etudes superieurs de l'Universite de Montreal MOP-152556 / Canadian Institutes of Health Research; Canadian Institutes of Health Research (CIHR) Canada Research Chairs Program; Canada Research Chairs
- Language
- English
- Date published
- 11/01/2009
- Academic Unit
- Surgery
- Record Identifier
- 9984322954102771
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