A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length

Syed H Askree; Tal Yehuda; Sarit Smolikov; Raya Gurevich; Joshua Hawk; Carrie Coker; Anat Krauskopf; Martin Kupiec; Michael J McEachern

doi:10.1073/pnas.0401263101

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A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length

Journal article

Open access

Peer reviewed

A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length

Syed H Askree, Tal Yehuda, Sarit Smolikov, Raya Gurevich, Joshua Hawk, Carrie Coker, Anat Krauskopf, Martin Kupiec and Michael J McEachern

Proceedings of the National Academy of Sciences - PNAS, Vol.101(23), pp.8658-8663

06/08/2004

DOI: 10.1073/pnas.0401263101

PMCID: PMC423251

PMID: 15161972

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Abstract

Telomeres are nucleoprotein structures present at the ends of eukaryotic chromosomes that play a central role in guarding the integrity of the genome by protecting chromosome ends from degradation and fusion. Length regulation is central to telomere function. To broaden our knowledge about the mechanisms that control telomere length, we have carried out a systematic examination of approximately 4,800 haploid deletion mutants of Saccharomyces cerevisiae for telomere-length alterations. By using this screen, we have identified >150 candidate genes not previously known to affect telomere length. In two-thirds of the identified mutants, short telomeres were observed; whereas in one-third, telomeres were lengthened. The genes identified are very diverse in their functions, but certain categories, including DNA and RNA metabolism, chromatin modification, and vacuolar traffic, are overrepresented. Our results greatly enlarge the number of known genes that affect telomere metabolism and will provide insights into how telomere function is linked to many other cellular processes.

Phenotype

DNA, Fungal - genetics

Genome, Fungal

Saccharomyces cerevisiae - genetics

Base Sequence

Gene Deletion

Mutation

Telomere - genetics

Details

Title: Subtitle: A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length
Creators: Syed H Askree - Department of Genetics, University of Georgia, Athens, GA 30602, USA
Tal Yehuda
Sarit Smolikov
Raya Gurevich
Joshua Hawk
Carrie Coker
Anat Krauskopf
Martin Kupiec
Michael J McEachern
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.101(23), pp.8658-8663
DOI: 10.1073/pnas.0401263101
PMID: 15161972
PMCID: PMC423251
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: United States
Grant note: GM61645-01 / NIGMS NIH HHS R01 GM061645 / NIGMS NIH HHS
Language: English
Date published: 06/08/2004
Academic Unit: Biology
Record Identifier: 9983991991502771

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