A humanized neutralizing antibody against MERS-CoV targeting the receptor-binding domain of the spike protein

Yan Li; Yuhua Wan; Peipei Liu; Jincun Zhao; Guangwen Lu; Jianxun Qi; Qihui Wang; Xuancheng Lu; Ying Wu; Wenjun Liu; Buchang Zhang; Kwok-Yung Yuen; Stanley Perlman; George F Gao; Jinghua Yan

doi:10.1038/cr.2015.113

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A humanized neutralizing antibody against MERS-CoV targeting the receptor-binding domain of the spike protein

Journal article

Open access

Peer reviewed

A humanized neutralizing antibody against MERS-CoV targeting the receptor-binding domain of the spike protein

Yan Li, Yuhua Wan, Peipei Liu, Jincun Zhao, Guangwen Lu, Jianxun Qi, Qihui Wang, Xuancheng Lu, Ying Wu, Wenjun Liu, …

Cell research, Vol.25(11), pp.1237-1249

11/2015

DOI: 10.1038/cr.2015.113

PMCID: PMC4650419

PMID: 26391698

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Abstract

The newly-emerging Middle East respiratory syndrome coronavirus (MERS-CoV) can cause severe and fatal acute respiratory disease in humans. Despite global efforts, the potential for an associated pandemic in the future cannot be excluded. The development of effective counter-measures is urgent. MERS-CoV-specific anti-viral drugs or vaccines are not yet available. Using the spike receptor-binding domain of MERS-CoV (MERS-RBD) to immunize mice, we identified two neutralizing monoclonal antibodies (mAbs) 4C2 and 2E6. Both mAbs potently bind to MERS-RBD and block virus entry in vitro with high efficacy.

Antibodies, Neutralizing - administration & dosage

Coronavirus Infections - drug therapy

Middle East Respiratory Syndrome Coronavirus - drug effects

Spike Glycoprotein, Coronavirus - therapeutic use

Antibodies, Neutralizing - immunology

Animals

Antibodies, Monoclonal, Humanized - administration & dosage

Antibodies, Neutralizing - chemistry

Antibodies, Viral - chemistry

Antibodies, Viral - immunology

Spike Glycoprotein, Coronavirus - immunology

Mice

Mice, Inbred BALB C

Antibodies, Monoclonal, Humanized - immunology

Antibodies, Viral - administration & dosage

Antibodies, Monoclonal, Humanized - chemistry

Details

Title: Subtitle: A humanized neutralizing antibody against MERS-CoV targeting the receptor-binding domain of the spike protein
Creators: Yan Li - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Academy of Sciences, Beijing 100101, China
Yuhua Wan - CAS Key Laboratory of Microbial and Metabolic engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Peipei Liu - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Academy of Sciences, Beijing 100101, China
Jincun Zhao - State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, China
Guangwen Lu - State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan 610041, China
Jianxun Qi - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Academy of Sciences, Beijing 100101, China
Qihui Wang - CAS Key Laboratory of Microbial and Metabolic engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Xuancheng Lu - Laboratory Animal Center, Chinese Center for Disease Control and Prevention (China CDC), Beijing 102206, China
Ying Wu - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Academy of Sciences, Beijing 100101, China
Wenjun Liu - CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Academy of Sciences, Beijing 100101, China
Buchang Zhang - School of Life Sciences, Anhui University, Hefei, Anhui 230039, China
Kwok-Yung Yuen - State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
Stanley Perlman - Departments of Microbiology and Pediatrics, University of Iowa, Iowa City, IA 52242, USA
George F Gao - Office of Director-General, Chinese Center for Disease Control and Prevention (China CDC), Beijing 102206, China
Jinghua Yan - CAS Key Laboratory of Microbial and Metabolic engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Resource Type: Journal article
Publication Details: Cell research, Vol.25(11), pp.1237-1249
DOI: 10.1038/cr.2015.113
PMID: 26391698
PMCID: PMC4650419
NLM abbreviation: Cell Res
ISSN: 1001-0602
eISSN: 1748-7838
Publisher: England
Grant note: P01 AI060699 / NIAID NIH HHS AI60699 / NIAID NIH HHS R01 AI091322 / NIAID NIH HHS
Language: English
Date published: 11/2015
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777470902771

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