Journal article
A lipid-soluble iron chelator alters cell cycle regulatory protein binding in breast cancer cells compared to normal breast cells
Journal of experimental therapeutics & oncology, Vol.6(3), pp.193-200
01/01/2007
PMID: 17552359
Abstract
The lipid-soluble iron chelator desferri-exochelin (D-Exo) causes reversible cell cycle arrest in normal human mammary epithelial cells (NHMEC) but triggers apoptotic cell death in human breast cancer cells. We studied the effects of iron chelation with D-Exo on cell cycle regulatory proteins in cultures of NHMEC and MCF-7 breast cancer cells. In co-immunoprecipitation studies, D-Exo inhibited binding of cyclins A and E to cyclin dependent kinase 2 (CDK2) in NHMEC, but in MCF-7 cells binding of these cyclins to CDK2 was enhanced. D-Exo treatment markedly increased expression of p53 and increased binding of p21 to CDK2 in the MCF-7 cells but not in NHMEC. Therefore differences in effects of iron chelation on cell cycle protein binding in cancer cells compared to normal cells may trigger apoptosis in cancer cells while normal breast cells are spared.
Details
- Title: Subtitle
- A lipid-soluble iron chelator alters cell cycle regulatory protein binding in breast cancer cells compared to normal breast cells
- Creators
- Paula M. B. Pahl - Univ Colorado, Hlth Sci Ctr, Denver, CO 80262 USASara M. Reese - Univ Colorado, Hlth Sci Ctr, Denver, CO 80262 USALawrence D. Horwitz - Univ Colorado, Hlth Sci Ctr, Denver, CO 80262 USA
- Resource Type
- Journal article
- Publication Details
- Journal of experimental therapeutics & oncology, Vol.6(3), pp.193-200
- Publisher
- Old City Publishing Inc
- PMID
- 17552359
- ISSN
- 1359-4117
- eISSN
- 1533-869X
- Number of pages
- 8
- Grant note
- R01HL055291 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) HL55291 / NHLBI NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI)
- Language
- English
- Date published
- 01/01/2007
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984656533002771
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