A method for analyzing AFM force mapping data obtained from soft tissue cryosections

Cydney A. Wong; Nina Sara Fraticelli Guzmán; A. Thomas Read; Adam Hedberg-Buenz; Michael G. Anderson; Andrew J. Feola; Todd Sulchek; C. Ross Ethier

doi:10.1016/j.jbiomech.2024.112113

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A method for analyzing AFM force mapping data obtained from soft tissue cryosections

Journal article

Peer reviewed

A method for analyzing AFM force mapping data obtained from soft tissue cryosections

Cydney A. Wong, Nina Sara Fraticelli Guzmán, A. Thomas Read, Adam Hedberg-Buenz, Michael G. Anderson, Andrew J. Feola, Todd Sulchek and C. Ross Ethier

Journal of biomechanics, Vol.168, 112113

05/01/2024

DOI: 10.1016/j.jbiomech.2024.112113

PMCID: PMC11128031

PMID: 38648717

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Abstract

Atomic force microscopy (AFM) is a valuable tool for assessing mechanical properties of biological samples, but interpretations of measurements on whole tissues can be difficult due to the tissue’s highly heterogeneous nature. To overcome such difficulties and obtain more robust estimates of tissue mechanical properties, we describe an AFM force mapping and data analysis pipeline to characterize the mechanical properties of cryosectioned soft tissues. We assessed this approach on mouse optic nerve head and rat trabecular meshwork, cornea, and sclera. Our data show that the use of repeated measurements, outlier exclusion, and log-normal data transformation increases confidence in AFM mechanical measurements, and we propose that this methodology can be broadly applied to measuring soft tissue properties from cryosections.

Details

Title: Subtitle: A method for analyzing AFM force mapping data obtained from soft tissue cryosections
Creators: Cydney A. Wong - The Wallace H. Coulter Department of Biomedical Engineering
Nina Sara Fraticelli Guzmán - Georgia Institute of Technology
A. Thomas Read - The Wallace H. Coulter Department of Biomedical Engineering
Adam Hedberg-Buenz - University of Iowa
Michael G. Anderson - University of Iowa
Andrew J. Feola - Emory University
Todd Sulchek - Georgia Institute of Technology
C. Ross Ethier - The Wallace H. Coulter Department of Biomedical Engineering
Resource Type: Journal article
Publication Details: Journal of biomechanics, Vol.168, 112113
DOI: 10.1016/j.jbiomech.2024.112113
PMID: 38648717
PMCID: PMC11128031
NLM abbreviation: J Biomech
ISSN: 0021-9290
eISSN: 1873-2380
Publisher: Elsevier Ltd
Language: English
Date published: 05/01/2024
Academic Unit: Molecular Physiology and Biophysics; Ophthalmology and Visual Sciences
Record Identifier: 9984618627302771

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