Journal article
A method for detailed movement pattern analysis of tadpole startle response
Journal of the Experimental Analysis of Behavior, Vol.108(1), pp.113-124
07/01/2017
DOI: 10.1002/jeab.263
PMCID: PMC5705052
PMID: 28653338
Abstract
Prolonged space flight, specifically microgravity, presents a problem for space exploration. Animal models with altered connections of the vestibular ear, and thus altered gravity sensation, would allow the examination of the effects of microgravity and how various countermeasures can establish normal function. We describe an experimental apparatus to monitor the effects of ear manipulations to generate asymmetric gravity input on the tadpole escape response. To perform the movement pattern analysis, an imaging apparatus was developed that uses a high-speed camera to obtain time-resolved, high-resolution images of tadpole movements. Movements were recorded in a temperature-controlled test chamber following mechanical stimulation with a solenoid actuator, to elicit a C-start response. Temperature within the test cell was controlled with a recirculating water bath. Xenopus laevis embryos were obtained using a standard fertilization technique. Tadpole response to a controlled perturbation was recorded in unprecedented detail and the approach was validated by describing the distinct differences in response between normal and one-eared tadpoles. The experimental apparatus and methods form an important element of a rigorous investigation into the response of the tadpole vestibular system to mechanical and biochemical manipulations, and can ultimately contribute to improved understanding of the effects of altered gravity perception on humans.
Details
- Title: Subtitle
- A method for detailed movement pattern analysis of tadpole startle response
- Creators
- Kasra ZareiKaren L ElliottSanam ZareiBernd Fritzsch - University of Iowa, BiologyJames H J Buchholz - University of Iowa, Mechanical Engineering
- Resource Type
- Journal article
- Publication Details
- Journal of the Experimental Analysis of Behavior, Vol.108(1), pp.113-124
- DOI
- 10.1002/jeab.263
- PMID
- 28653338
- PMCID
- PMC5705052
- NLM abbreviation
- J Exp Anal Behav
- ISSN
- 0022-5002
- eISSN
- 1938-3711
- Publisher
- Blackwell Publishing Ltd; Malden
- Grant note
- DOI: 10.13039/100000104, name: National Aeronautics and Space Administration, award: NNX10AK63H; DOI: 10.13039/100005740, name: Iowa Space Grant Consortium
- Language
- English
- Date published
- 07/01/2017
- Academic Unit
- Biology; Industrial and Systems Engineering; Craniofacial Anomalies Research Center
- Record Identifier
- 9983763591302771
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