A method for freezing synchronous mitotic and G1 cells

M. J BORRELLI; M. A MACKEY; W. C DEWEY

doi:10.1016/0014-4827(87)90313-2

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A method for freezing synchronous mitotic and G1 cells

Journal article

Peer reviewed

A method for freezing synchronous mitotic and G1 cells

M. J BORRELLI, M. A MACKEY and W. C DEWEY

Experimental cell research, Vol.170(2), pp.363-368

1987

DOI: 10.1016/0014-4827(87)90313-2

PMID: 3595736

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Abstract

A modification of the protocol developed by Kawamoto, J C & Barrett, J N, Brain res (1986), in press [3] for freezing primary neuron cultures in a solution containing low sodium and high lactate and potassium concentrations was used to freeze synchronous mitotic and G1 CHO cells. After thawing, the cells behaved as if they had never been frozen with respect to cell growth, cell division, plating efficiency, and hyperthermic sensitivity.

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Diverse techniques

Molecular and cellular biology

Details

Title: Subtitle: A method for freezing synchronous mitotic and G1 cells
Creators: M. J BORRELLI - Univ. California, radiation oncology res. lab., San Francisco CA 94143, United States
M. A MACKEY - Univ. California, radiation oncology res. lab., San Francisco CA 94143, United States
W. C DEWEY - Univ. California, radiation oncology res. lab., San Francisco CA 94143, United States
Resource Type: Journal article
Publication Details: Experimental cell research, Vol.170(2), pp.363-368
Publisher: Elsevier; Orlando, FL
DOI: 10.1016/0014-4827(87)90313-2
PMID: 3595736
ISSN: 0014-4827
eISSN: 1090-2422
Language: English
Date published: 1987
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Radiology
Record Identifier: 9984064227802771

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