Journal article
A method for plasmid purification directly from yeast
Analytical biochemistry, Vol.307(1), pp.13-17
2002
DOI: 10.1016/S0003-2697(02)00018-0
PMID: 12137773
Abstract
A rapid technique for purifying plasmids from yeast
Saccharomyces cerevisiae is described that yields high-quality DNA suitable for bacterial transformation, yeast transformation, and direct DNA sequencing. The method requires only small culture volumes and proprietary bacterial plasmid miniprep kits that allow one to simultaneously prepare a large number of samples in a very short period of time while avoiding the use of toxic organic chemicals. Both yeast single-copy CEN/ARS and high-copy 2μ shuttle plasmids can be isolated using this method. This technique is useful for plasmid purification from yeast two-hybrid experiments as well as yeast genetics and molecular biology experiments.
Details
- Title: Subtitle
- A method for plasmid purification directly from yeast
- Creators
- Madhu V SinghP Anthony Weil
- Resource Type
- Journal article
- Publication Details
- Analytical biochemistry, Vol.307(1), pp.13-17
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0003-2697(02)00018-0
- PMID
- 12137773
- ISSN
- 0003-2697
- eISSN
- 1096-0309
- Language
- English
- Date published
- 2002
- Academic Unit
- Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094316502771
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