Journal article
A multi-center ring trial of allergen analysis using fluorescent multiplex array technology
Journal of immunological methods, Vol.387(1-2), pp.89-95
01/31/2013
DOI: 10.1016/j.jim.2012.09.015
PMCID: PMC3955085
PMID: 23085532
Abstract
Consistent performance of allergen assays is essential to ensure reproducibility of exposure assessments for investigations of asthma and occupational allergic disease. This study evaluated intra- and inter-laboratory reproducibility of a fluorescent multiplex array, which simultaneously measures eight indoor allergens in a single reaction well.
A multi-center study was performed in nine laboratories in the US and Europe to determine the inter-laboratory variability of an 8-plex array for dust mite, cat, dog, rat, mouse and cockroach allergens. Aliquots of 151 dust extract samples were sent to participating centers and analyzed by each laboratory on three separate occasions. Agreement within and between laboratories was calculated by the concordance correlation coefficient (CCC).
Results were obtained for over 32,000 individual allergen measurements. Levels covered a wide range for all allergens from below the lower limit of detection (LLOD=0.1–9.8ng/ml) to higher than 6800ng/ml for all allergens except Mus m 1, which was up to 1700ng/ml. Results were reproducible within as well as between laboratories. Within laboratories, 94% of CCC were ≥0.90, and 80% of intra-laboratory results fell within a 10% coefficient of variance (CV%). Results between laboratories also showed highly significant positive correlations for all allergens (~0.95, p<0.001). Overall means of results were comparable, and inter-laboratory CV% for all allergens except Rat n 1 ranged between 17.6% and 26.6%.
The data indicate that performance criteria for fluorescent multiplex array technology are reproducible within and between laboratories. Multiplex technology provides standardized and consistent allergen measurements that will streamline environmental exposure assessments in allergic disease.
► Reproducibility of a multiplex array for allergen measurement was evaluated. ► The international ring trial included nine US and European laboratories. ► Each center analyzed 151 dust extract samples for eight allergens on three occasions. ► The array produced data that were reproducible both within and between laboratories.
Details
- Title: Subtitle
- A multi-center ring trial of allergen analysis using fluorescent multiplex array technology
- Creators
- Eva M King - INDOOR Biotechnologies Inc., Charlottesville, VA, USAStephanie Filep - INDOOR Biotechnologies Inc., Charlottesville, VA, USABryan Smith - INDOOR Biotechnologies Inc., Charlottesville, VA, USAThomas Platts-Mills - University of Virginia, Allergy Division, Charlottesville VA, USARobert G Hamilton - Johns Hopkins University, School of Medicine, Baltimore, MD, USADetlef Schmechel - NIOSH/CDC, Morgantown, WV, USAJoanne E Sordillo - Channing Laboratory, Dept of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USADonald Milton - Maryland Institute of Applied Environmental Health, University of Maryland, College Park, MD, USARonald van Ree - Academic Medical Center, Dept of Experimental Immunology and Otorhinolaryngology, Amsterdam, The NetherlandsEsmeralda J.M Krop - Institute for Risk Assessment Sciences, Div. Environmental Epidemiology, Utrecht University, The NetherlandsDick J.J Heederik - Institute for Risk Assessment Sciences, Div. Environmental Epidemiology, Utrecht University, The NetherlandsNervana Metwali - Dept of Occupational and Environmental Health, University of Iowa, Iowa City, IA, USAPeter S Thorne - Dept of Occupational and Environmental Health, University of Iowa, Iowa City, IA, USADarryl C Zeldin - Div. of Intramural Research, NIEHS, NIH, Research Triangle Park, NC, USAMichelle L Sever - Rho Inc., Chapel Hill, NC, USAAgustin Calatroni - Rho Inc., Chapel Hill, NC, USASamuel J Arbes - Rho Inc., Chapel Hill, NC, USAHerman E Mitchell - Rho Inc., Chapel Hill, NC, USAMartin D Chapman - INDOOR Biotechnologies Inc., Charlottesville, VA, USA
- Resource Type
- Journal article
- Publication Details
- Journal of immunological methods, Vol.387(1-2), pp.89-95
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.jim.2012.09.015
- PMID
- 23085532
- PMCID
- PMC3955085
- ISSN
- 0022-1759
- eISSN
- 1872-7905
- Grant note
- Intramural Research Program of the NIH ES55545C / National Institute of Environmental Health Sciences U.S. National Institutes of Health Small Business Innovation and Research (SBIR)
- Language
- English
- Date published
- 01/31/2013
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health
- Record Identifier
- 9983997460802771
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