A multi-chamber tissue culture device for load-dependent parallel evaluation of tendon explants

Endre Soreide; Janet M. Denbeigh; Eric A. Lewallen; Roman Thaler; Rebekah M. Samsonraj; Dakota L. Jones; Wei Xu; Dirk Larson; Lars Nordsletten; Sanjeev Kakar; Andre J. van Wijnen

doi:10.1186/s12891-019-2896-2

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A multi-chamber tissue culture device for load-dependent parallel evaluation of tendon explants

Journal article

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A multi-chamber tissue culture device for load-dependent parallel evaluation of tendon explants

Endre Soreide, Janet M. Denbeigh, Eric A. Lewallen, Roman Thaler, Rebekah M. Samsonraj, Dakota L. Jones, Wei Xu, Dirk Larson, Lars Nordsletten, Sanjeev Kakar, …

BMC musculoskeletal disorders, Vol.20(1), 549

11/18/2019

DOI: 10.1186/s12891-019-2896-2

PMCID: PMC6862789

PMID: 31739778

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Abstract

Background Injuries in the musculoskeletal system, such as tendon and ligament ruptures, are challenging to manage and often require surgical reconstructions with limited long-term success. Thus, characterizations of these tissues are urgently needed to better understand cellular mechanisms that regulate tissue homeostasis and healing. Explant culturing systems allow for ex vivo analysis of tissues in an environment that mimics the native microenvironment in vivo. Methods Collaborative efforts within our institution facilitated the establishment of a novel explant culturing system. Tissue specimens cultured in single wells, with individual applied loading and/or biological environment, allowed characterization of tissue cultured under a variety of biological loading conditions. Quantitative PCR analysis for selected gene markers was our primary outcome. Results Data were stratified for analysis by either culture environment or loading condition. Our gene expression results show that specimens clustered by culture condition may differ in molecular markers related to ECM production (e.g., Col1a1, Adamts4) and/or organization (e.g., Tnc, Dnc). In contrast, loading condition did significantly alter the median gene expression levels of tissues in comparison to unloaded control samples, although gene expression values related to ECM degradation (e.g., Mmp1, Mmp10) were altered in tendons cultured under tension in the device. Conclusion Our study demonstrates promising utility of a novel explant culturing system for further characterization of musculoskeletal tissues such as native tendons and ligaments, as well as pathologic fibrotic tissues resulting from arthrofibrosis or Dupuytren’s disease.

Details

Title: Subtitle: A multi-chamber tissue culture device for load-dependent parallel evaluation of tendon explants
Creators: Endre Soreide - Mayo Clinic
Janet M. Denbeigh - Mayo Clinic
Eric A. Lewallen - Mayo Clinic
Roman Thaler - Mayo Clinic
Rebekah M. Samsonraj - Mayo Clinic
Dakota L. Jones - Mayo Clinic
Wei Xu - Mayo Clinic
Dirk Larson - Mayo Clinic
Lars Nordsletten - Oslo University Hospital
Sanjeev Kakar - Mayo Clinic
Andre J. van Wijnen - Mayo Clinic
Resource Type: Journal article
Publication Details: BMC musculoskeletal disorders, Vol.20(1), 549
DOI: 10.1186/s12891-019-2896-2
PMID: 31739778
PMCID: PMC6862789
NLM abbreviation: BMC Musculoskelet Disord
ISSN: 1471-2474
eISSN: 1471-2474
Publisher: BioMed Central
Grant note: R01 AR049069 / ; 239871/F20 / ;
Language: English
Date published: 11/18/2019
Academic Unit: Anatomy and Cell Biology
Record Identifier: 9984949240202771

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