A multifunctional micropore-forming bioink with enhanced anti-bacterial and anti-inflammatory properties

Mian Wang; Wanlu Li; Zeyu Luo; Guosheng Tang; Xuan Mu; Xiao Kuang; Jie Guo; Zhibo Zhao; Regina Sanchez Flores; Zewei Jiang; Liming Lian; Julia Olga Japo; Amir M Ghaemmaghami; Yu Shrike Zhang

doi:10.1088/1758-5090/ac5936

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A multifunctional micropore-forming bioink with enhanced anti-bacterial and anti-inflammatory properties

Journal article

Peer reviewed

A multifunctional micropore-forming bioink with enhanced anti-bacterial and anti-inflammatory properties

Mian Wang, Wanlu Li, Zeyu Luo, Guosheng Tang, Xuan Mu, Xiao Kuang, Jie Guo, Zhibo Zhao, Regina Sanchez Flores, Zewei Jiang, …

Biofabrication, Vol.14(2), p.24105

03/11/2022

DOI: 10.1088/1758-5090/ac5936

PMCID: PMC8962756

PMID: 35226880

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Abstract

Three-dimensional (3D) bioprinting has emerged as an enabling tool for various biomedical applications, such as tissue regeneration and tissue model engineering. To this end, the development of bioinks with multiple functions plays a crucial role in the applications of 3D bioprinting technologies. In this study, we propose a new bioink based on two immiscible aqueous phases of gelatin methacryloyl (GelMA) and dextran, further endowed with anti-bacterial and anti-inflammatory properties. This micropore-forming GelMA-dextran (PGelDex) bioink exhibited excellent printability with vat-polymerization, extrusion, and handheld bioprinting methods. The porous structure was confirmed after bioprinting, which promoted the spreading of the encapsulated cells, exhibiting the exceptional cytocompatibility of this bioink formulation. To extend the applications of such a micropore-forming bioink, interleukin-4 (IL-4)-loaded silver-coated gold nanorods (AgGNRs) and human mesenchymal stem cells (MSCs) were simultaneously incorporated, to display synergistic anti-infection behavior and immunomodulatory function. The results revealed the anti-bacterial properties of the AgGNR-loaded PGelDex bioink for both Gram-negative and Gram-positive bacteria. The data also indicated that the presence of IL-4 and MSCs facilitated macrophage M2-phenotype differentiation, suggesting the potential anti-inflammatory feature of the bioink. Overall, this unique anti-bacterial and immunomodulatory micropore-forming bioink offers an effective strategy for the inhibition of bacterial-induced infections as well as the ability of immune-regulation, which is a promising candidate for broadened tissue bioprinting applications.

Anti-Inflammatory Agents

Bioprinting - methods

Dextrans

Gelatin - chemistry

Gelatin - pharmacology

Hydrogels - chemistry

Interleukin-4

Methacrylates

Printing, Three-Dimensional

Tissue Engineering - methods

Tissue Scaffolds - chemistry

Details

Title: Subtitle: A multifunctional micropore-forming bioink with enhanced anti-bacterial and anti-inflammatory properties
Creators: Mian Wang - Harvard University
Wanlu Li - Harvard University
Zeyu Luo - Harvard University
Guosheng Tang - Harvard University
Xuan Mu - Harvard University
Xiao Kuang - Harvard University
Jie Guo - Harvard University
Zhibo Zhao - Harvard University
Regina Sanchez Flores - Harvard University
Zewei Jiang - Harvard University
Liming Lian - Harvard University
Julia Olga Japo - Harvard University
Amir M Ghaemmaghami - University of Nottingham
Yu Shrike Zhang - Harvard University
Resource Type: Journal article
Publication Details: Biofabrication, Vol.14(2), p.24105
DOI: 10.1088/1758-5090/ac5936
PMID: 35226880
PMCID: PMC8962756
NLM abbreviation: Biofabrication
ISSN: 1758-5082
eISSN: 1758-5090
Grant note: R21 EB025270 / NIBIB NIH HHS R01 EB028143 / NIBIB NIH HHS R01 GM134036 / NIGMS NIH HHS R00 CA201603 / NCI NIH HHS R21 EB026175 / NIBIB NIH HHS
Language: English
Date published: 03/11/2022
Academic Unit: Roy J. Carver Department of Biomedical Engineering
Record Identifier: 9984277262502771

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