A naturally occurring human RPA subunit homolog does not support DNA replication or cell-cycle progression

Stuart J Haring; Troy D Humphreys; Marc S Wold

doi:10.1093/nar/gkp1062

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A naturally occurring human RPA subunit homolog does not support DNA replication or cell-cycle progression

Journal article

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A naturally occurring human RPA subunit homolog does not support DNA replication or cell-cycle progression

Stuart J Haring, Troy D Humphreys and Marc S Wold

Nucleic acids research, Vol.38(3), pp.846-858

01/2010

DOI: 10.1093/nar/gkp1062

PMCID: PMC2817474

PMID: 19942684

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Abstract

Replication Protein A (RPA) is a single-stranded DNA-binding protein essential for DNA replication, repair, recombination and cell-cycle regulation. A human homolog of the RPA2 subunit, called RPA4, was previously identified and shown to be expressed in colon mucosal and placental cells; however, the function of RPA4 was not determined. To examine the function of RPA4 in human cells, we carried out knockdown and replacement studies to determine whether RPA4 can substitute for RPA2 in the cell. Unlike RPA2, exogenous RPA4 expression did not support chromosomal DNA replication and lead to cell-cycle arrest in G2/M. In addition, RPA4 localized to sites of DNA repair and reduced γ-H2AX caused by RPA2 depletion. These studies suggest that RPA4 cannot support cell proliferation but can support processes that maintain the genomic integrity of the cell.

Genome Integrity, Repair and

Details

Title: Subtitle: A naturally occurring human RPA subunit homolog does not support DNA replication or cell-cycle progression
Creators: Stuart J Haring - Department of Biochemistry, University of Iowa, Carver College of Medicine, Iowa City, IA 52242, USA
Troy D Humphreys - Department of Biochemistry, University of Iowa, Carver College of Medicine, Iowa City, IA 52242, USA
Marc S Wold - Department of Biochemistry, University of Iowa, Carver College of Medicine, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Nucleic acids research, Vol.38(3), pp.846-858
DOI: 10.1093/nar/gkp1062
PMID: 19942684
PMCID: PMC2817474
NLM abbreviation: Nucleic Acids Res
ISSN: 0305-1048
eISSN: 1362-4962
Publisher: Oxford University Press
Language: English
Date published: 01/2010
Academic Unit: Radiation Oncology; Biochemistry and Molecular Biology
Record Identifier: 9984024514402771

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