Journal article
A neuropeptide ligand of the G protein-coupled receptor GPR103 regulates feeding, behavioral arousal, and blood pressure in mice
Proceedings of the National Academy of Sciences - PNAS, Vol.103(19), pp.7438-7443
04/28/2006
DOI: 10.1073/pnas.0602371103
PMCID: PMC1464357
PMID: 16648250
Abstract
Here, we report the isolation and characterization of an endogenous peptide ligand of GPR103 from rat brains. The purified peptide was found to be the 43-residue RF-amide peptide QRFP. We also describe two mouse homologues of human GPR103, termed mouse GPR103A and GPR103B. QRFP binds and activates the human GPR103, as well as mouse GPR103A and GPR103B, with nanomolar affinities in transfected cells. Systematic
in situ
hybridization analysis in mouse brains showed that QRFP is expressed exclusively in the periventricular and lateral hypothalamus, whereas the two receptor mRNAs are distinctly localized in various brain areas without an overlap to each other. When administered centrally in mice, QRFP induced feeding behavior, accompanied by increased general locomotor activity and metabolic rate. QRFP-induced food intake was abolished by preadministration of BIBP3226, a specific antagonist for the Y1 neuropeptide Y receptor. Hypothalamic prepro-QRFP mRNA expression was up-regulated upon fasting and in genetically obese
ob
/
ob
and
db
/
db
mice. Central QRFP administration also evoked highly sustained elevation of blood pressure and heart rate. Our findings suggest that QRFP and GPR103A/B may regulate diverse neuroendocrine and behavioral functions and implicate this neuropeptide system in metabolic syndrome.
Details
- Title: Subtitle
- A neuropeptide ligand of the G protein-coupled receptor GPR103 regulates feeding, behavioral arousal, and blood pressure in mice
- Creators
- Shinobu Takayasu - Hirosaki UniversityTakeshi Sakurai - University of TsukubaSatoshi Iwasaki - Japan Science and Technology AgencyHitoshi Teranishi - The University of TokyoAkihiro Yamanaka - University of TsukubaS. Clay Williams - The University of Texas Southwestern Medical CenterHaruhisa Iguchi - Japan Science and Technology AgencyYuka Imamura Kawasawa - Japan Science and Technology AgencyYukio Ikeda - Japan Science and Technology AgencyIori Sakakibara - The University of TokyoKousaku Ohno - University of TsukubaRyoichi X. Ioka - Japan Science and Technology AgencySaori Murakami - Japan Science and Technology AgencyNaoshi Dohmae - RIKENJian Xie - The University of Texas Southwestern Medical CenterToshihiro Suda - Hirosaki UniversityToshiyuki Motoike - The University of Texas Southwestern Medical CenterTakashi Ohuchi - Japan Science and Technology AgencyMasashi Yanagisawa - Czech Academy of Sciences, Institute of Molecular GeneticsJuro Sakai - The University of Tokyo
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.103(19), pp.7438-7443
- Publisher
- National Academy of Sciences
- DOI
- 10.1073/pnas.0602371103
- PMID
- 16648250
- PMCID
- PMC1464357
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Language
- English
- Date published
- 04/28/2006
- Academic Unit
- Nephrology; Internal Medicine
- Record Identifier
- 9984360054302771
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