Journal article
A new electrochemical HPLC method for analysis of enkephalins and endomorphins
Journal of neuroscience methods, Vol.150(1), pp.74-79
2006
DOI: 10.1016/j.jneumeth.2005.06.001
PMID: 16051372
Abstract
Endogenous opioid peptides, enkephalins and endomorphins, are located in key regions involved in pain transmission and analgesia, including the spinal cord. These endogenous peptides activate opioid receptors to produce analgesia and reduce pain. We describe a new method to measure enkephalin and endomorphins by high performance liquid chromatography with electrochemical detection. This method allows use of a small sample volume to measure met-enkephalin, leu-enkephalin, endomorphin-1 and endomorphin-2 simultaneously. Using push–pull perfusion of the spinal cord, there were detectable concentrations of met-enkephalin, leu-enkephalin, and endomorphin-2. Further infusion of 100
mM potassium chloride evoked release of met-enkephalin and endomorphin-2 but not leu-enkephalin. Thus, we have developed a method to simultaneously measure enkephalins and endomorphins in small sample volume that allows measurement of these opioid peptides in vivo.
Details
- Title: Subtitle
- A new electrochemical HPLC method for analysis of enkephalins and endomorphins
- Creators
- Tammy L LisiKathleen A Sluka
- Resource Type
- Journal article
- Publication Details
- Journal of neuroscience methods, Vol.150(1), pp.74-79
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.jneumeth.2005.06.001
- PMID
- 16051372
- ISSN
- 0165-0270
- eISSN
- 1872-678X
- Language
- English
- Date published
- 2006
- Academic Unit
- Iowa Neuroscience Institute; Nursing; Physical Therapy and Rehabilitation Science; Neuroscience and Pharmacology
- Record Identifier
- 9984040348502771
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