A new humanized HLA transgenic mouse model of multiple sclerosis expressing class II on mouse CD4 T cells

Ashutosh Mangalam; Moses Rodriguez; Chella David

doi:10.1196/annals.1394.005

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A new humanized HLA transgenic mouse model of multiple sclerosis expressing class II on mouse CD4 T cells

Journal article

Open access

Peer reviewed

A new humanized HLA transgenic mouse model of multiple sclerosis expressing class II on mouse CD4 T cells

Ashutosh Mangalam, Moses Rodriguez and Chella David

Annals of the New York Academy of Sciences, Vol.1103(1), pp.112-117

04/2007

DOI: 10.1196/annals.1394.005

PMCID: PMC2266826

PMID: 17376829

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http://doi.org/10.1196/annals.1394.005View

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Abstract

Among all the genetic factors associated with susceptibility to multiple sclerosis (MS), the strongest association has been seen with expression of certain major histocompatibility complex (MHC) class II molecules, although analysis of their exact function remains complicated. In general, the expression of class II is restricted to professional antigen-presenting cells; however, human but not mouse CD4+ T cells express class II on their surface. Functional studies of class II+ CD4+ T cells have been hampered due to lack of a proper animal model. Here, we describe development and characterization of a new humanized class II transgenic (tg) mouse expressing HLA-DR3 on mouse endogenous class II-negative background.

HLA-D Antigens - genetics

Lymphocyte Activation

Humans

HLA Antigens - immunology

Mice, Transgenic

HLA Antigens - genetics

Antigen-Presenting Cells - immunology

Multiple Sclerosis - classification

CD4-Positive T-Lymphocytes - immunology

Animals

Flow Cytometry

Multiple Sclerosis - immunology

T-Lymphocytes - immunology

Mice

Details

Title: Subtitle: A new humanized HLA transgenic mouse model of multiple sclerosis expressing class II on mouse CD4 T cells
Creators: Ashutosh Mangalam - Department of Immunology, Mayo Clinic College of Medicine, 200 1st Street SW, Rochester, MN 55905, USA. mangalam.ashutosh@mayo.edu
Moses Rodriguez
Chella David
Resource Type: Journal article
Publication Details: Annals of the New York Academy of Sciences, Vol.1103(1), pp.112-117
DOI: 10.1196/annals.1394.005
PMID: 17376829
PMCID: PMC2266826
NLM abbreviation: Ann N Y Acad Sci
ISSN: 0077-8923
eISSN: 1749-6632
Publisher: United States
Grant note: R01 NS052173 / NINDS NIH HHS R01 NS052173-02 / NINDS NIH HHS
Language: English
Date published: 04/2007
Academic Unit: Pathology; Iowa Neuroscience Institute
Record Identifier: 9983905528502771

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