Journal article
A new method for delivering alkanes to mammalian cells: preparation and preliminary characterization of an inclusion complex between β-cyclodextrin and pristane (2,6,10,14-tetramethylpentadecane)
Toxicology (Amsterdam), Vol.69(3), pp.301-315
1991
DOI: 10.1016/0300-483X(91)90189-8
PMID: 1949053
Abstract
Pristane (2,6,10,14-teramethylpentadecane) is an isoalkane which induces plasma cell tumorigenesis in genetically susceptible strains of mice. Attempts to study the biological activity of pristane on cells in vitro have been hindered by the extreme hydrophobicity and hence complete immiscibility of the compound in aqueous cell culture media. In comparing different solubilization protocols such as using organic solvents, liposomes, and molecular encapsulation into β-cyclodextrin (β-CyD), it was found that β-CyD/pristane inclusion complexes were optimal for delivery of the hydrocarbon to cells. After solubilization in β-CyD, pristane was cytotoxic (
51Cr release assay) to murine B lymphocyte lines in culture (P388, NSF-1, and SJL-4) and inhibited the lipopolysaccharide-induced stimulation of splenic B lymphocyte proliferation and blast formation (Coulter counter analysis) when added in the micromolar concentration range. Moreover, sub-toxic concentrations of β-CyD/pristane inclusion complexes were found to have a small but reproducible mitogenic effect ([
3H]thymidine incorporation) on SJL-4 and 308 (murine initiated keratinocyte) cells in culture but not on P388 cells. The results indicate that molecular encapsulation of pristane into β-CyD provides a new and effective method for delivering low concentrations of alkanes to mammalian cells in vitro.
Details
- Title: Subtitle
- A new method for delivering alkanes to mammalian cells: preparation and preliminary characterization of an inclusion complex between β-cyclodextrin and pristane (2,6,10,14-tetramethylpentadecane)
- Creators
- Siegfried JanzEmily Shacter
- Resource Type
- Journal article
- Publication Details
- Toxicology (Amsterdam), Vol.69(3), pp.301-315
- Publisher
- Elsevier Ireland Ltd
- DOI
- 10.1016/0300-483X(91)90189-8
- PMID
- 1949053
- ISSN
- 0300-483X
- eISSN
- 1879-3185
- Language
- English
- Date published
- 1991
- Academic Unit
- Pathology
- Record Identifier
- 9984083828902771
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