Journal article
A novel assay of biofilm antifungal activity reveals that amphotericin B and caspofungin lyse Candida albicans cells in biofilms
Yeast (Chichester, England), Vol.28(8), pp.561-568
08/2011
DOI: 10.1002/yea.1860
PMID: 21674619
Abstract
The ability of Candida albicans to form drug-resistant biofilms is an important factor in its contribution to human disease. Assays to identify and characterize molecules with activity against fungal biofilms are crucial for the development of drugs with improved anti-biofilm activity. Here we report the application of an adenylate kinase (AK)-based cytotoxicity assay of fungal cell lysis to the characterization of agents active against C. albicans biofilms. We have developed three protocols for the AK assay. The first measures AK activity in the supernatants of biofilms treated with antifungal drugs and can be performed in parallel with a standard 2,3-bis-(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-caboxanilide-based biofilm susceptibility assay; a second, more sensitive protocol measures the AK activity present within the biofilm matrix; and a third procedure allows the direct visualization of lytic activity toward biofilms formed on catheter material. Amphotericin B and caspofungin, the two most effective anti-biofilm drugs currently used to treat fungal infections, both directly lyse planktonic C. albicans cells in vitro, leading to the release of AK into the culture medium. These studies serve to validate the AK-based lysis assay as a useful addition to the methods for the characterization of antifungal agents active toward biofilms and provide insights into the mode of action of amphotericin B and caspofungin against C. albicans biofilms.
Details
- Title: Subtitle
- A novel assay of biofilm antifungal activity reveals that amphotericin B and caspofungin lyse Candida albicans cells in biofilms
- Creators
- Louis DiDone - Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, NY, USADuana OgaDamian J Krysan
- Resource Type
- Journal article
- Publication Details
- Yeast (Chichester, England), Vol.28(8), pp.561-568
- DOI
- 10.1002/yea.1860
- PMID
- 21674619
- ISSN
- 0749-503X
- eISSN
- 1097-0061
- Grant note
- 1R01AI075033 / NIAID NIH HHS
- Language
- English
- Date published
- 08/2011
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093209602771
Metrics
24 Record Views