Journal article
A novel non-canonical function for IRF6 in the recycling of E-cadherin
Molecular biology of the cell, Vol.35(7), ar:102
07/01/2024
DOI: 10.1091/mbc.E23-11-0430
PMCID: PMC11244161
PMID: 38809584
Abstract
Interferon Regulatory Factor 6 (IRF6) is a transcription factor essential for keratinocyte cell-cell adhesions. Previously, we found that recycling of E-cadherin was defective in the absence of IRF6, yet total E-cadherin levels were not altered, suggesting a previously unknown, non-transcriptional function for IRF6. IRF6 protein contains a DNA binding domain (DBD) and a protein binding domain (PBD). The transcriptional function of IRF6 depends on its DBD and PBD, however, whether the PBD is necessary for the interaction with cytoplasmic proteins has yet to be demonstrated. Here, we show that an intact PBD is required for recruitment of cell-cell adhesion proteins at the plasma membrane, including the recycling of E-cadherin. Colocalizations and co-immunoprecipitations reveal that IRF6 forms a complex in recycling endosomes with Rab11, Myosin Vb and E-cadherin, and that the PBD is required for this interaction. These data indicate that IRF6 is a novel effector of the endosomal recycling of E-cadherin and demonstrate a non-transcriptional function for IRF6 in regulating cell-cell adhesions.
Details
- Title: Subtitle
- A novel non-canonical function for IRF6 in the recycling of E-cadherin
- Creators
- Angelo Antiguas - University of IowaMartine Dunnwald - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Molecular biology of the cell, Vol.35(7), ar:102
- DOI
- 10.1091/mbc.E23-11-0430
- PMID
- 38809584
- PMCID
- PMC11244161
- NLM abbreviation
- Mol Biol Cell
- eISSN
- 1939-4586
- Publisher
- American Society for Cell Biology (ASCB)
- Language
- English
- Electronic publication date
- 05/29/2024
- Date published
- 07/01/2024
- Academic Unit
- Anatomy and Cell Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984635159702771
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