Journal article
A novel p21WAF1/CIP1 transcript is highly dependent on p53 for its basal expression in mouse tissues
Oncogene, Vol.23(49), pp.8154-8157
10/21/2004
DOI: 10.1038/sj.onc.1207820
PMID: 15361845
Abstract
p21WAF1/CIP1 is an important transcriptional target of p53 and it plays a critical role in growth arrest after DNA damage. Here, we report the identification of a novel alternate mouse p21 transcript that is conserved in evolution. It differs from the classical p21WAF1/CIP1 transcript in the first exon, which is located at approximately 2.8 kb upstream of transcriptional start site of p21WAF1/CIP1 and is sandwiched between two p53 binding sites. This novel p21 transcript is present in most mouse tissues with highest levels of expression in the spleen. In contrast to the classical p21WAF1/CIP1 transcript, this new transcript is highly dependent on p53 for its basal expression, as evidenced by its absence in nearly all of p53-/- mouse tissues. This transcript is also absent at nonpermissive temperature in a 10-1 mouse cell line lacking endogenous p53 and harboring temperature-sensitive p53 mutant. However, this novel transcript is induced to appreciable levels in the presence of high p53 activity at the permissive temperature. Our data suggest that p53-dependent induction of p21 may be an additive effect conferred by individual increases in the alternate and classical p21 transcripts.
Details
- Title: Subtitle
- A novel p21WAF1/CIP1 transcript is highly dependent on p53 for its basal expression in mouse tissues
- Creators
- Andrei L Gartel - Department of Medicine, 840 S Wood St, University of Illinois at Chicago, Chicago, IL 60612, USA. atyner@uic.eduSenthil K RadhakrishnanMichael S SerfasYoung H KwonAngela L Tyner
- Resource Type
- Journal article
- Publication Details
- Oncogene, Vol.23(49), pp.8154-8157
- Publisher
- England
- DOI
- 10.1038/sj.onc.1207820
- PMID
- 15361845
- ISSN
- 0950-9232
- eISSN
- 1476-5594
- Grant note
- R01 DK056283-05 / NIDDK NIH HHS CA91146-01A1 / NCI NIH HHS DK56283 / NIDDK NIH HHS R01 DK056283 / NIDDK NIH HHS
- Language
- English
- Date published
- 10/21/2004
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9983979944902771
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