Journal article
A novel recombinant adeno-associated virus vector packaging system with HSV-1 amplicon providing helper functions
Science in China. Series C, Life sciences, Vol.42(5), pp.465-470
10/1999
DOI: 10.1007/BF02881769
PMID: 18726509
Abstract
A novel packaging system for producing recombinant adeno-associated virus (rAAV) vector was described. Instead of the conventional method for rAAV production by two-plasmid co-transfection followed by superinfection with adenovirus 5, an HSV-1 amplicon system expressing AAV-2 rep and cap genes from their native promoters was used to provide complete helper functions for rAAV replicating and packaging. This HSV-1 amplicon stock consisted of two kinds of infectious HSV-1 virions, a replicating-defective HSV-I amplicon pseudovirus harboring multi-copies of AAV-2 rep and cap gene and a temperature-sensitive HSV-1 mutant strain ts-KOS. High-titer rAAV was generated with this new packaging system. This packaging system gives a simple and scaleable process for rAAV production.
Details
- Title: Subtitle
- A novel recombinant adeno-associated virus vector packaging system with HSV-1 amplicon providing helper functions
- Creators
- Yuelong Shu - Beijing National Laboratory for Molecular SciencesXiaobing Wu - Beijing National Laboratory for Molecular SciencesTianzhong Yang - Beijing National Laboratory for Molecular SciencesHuiyu Gong - Beijing National Laboratory for Molecular SciencesYunde Hou - Beijing National Laboratory for Molecular SciencesZiying Yan - Beijing National Laboratory for Molecular Sciences
- Resource Type
- Journal article
- Publication Details
- Science in China. Series C, Life sciences, Vol.42(5), pp.465-470
- DOI
- 10.1007/BF02881769
- PMID
- 18726509
- ISSN
- 1006-9305
- eISSN
- 1862-2798
- Language
- English
- Date published
- 10/1999
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984284340302771
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