Journal article
A novel role for Bcl‐2 associated‐athanogene‐1 (Bag‐1) in regulation of the endoplasmic reticulum stress response in mammalian chondrocytes
Journal of cellular biochemistry, Vol.102(3), pp.786-800
10/15/2007
DOI: 10.1002/jcb.21328
PMID: 17546604
Abstract
BAG‐1 (Bcl‐2 associated athanogene‐1) is a multifunctional protein, linking cell proliferation, cell death, protein folding, and cell stress. In vivo, BAG‐1 is expressed in growth plate and articular cartilage, and the expression of BAG‐1 is decreased with aging. Chondrocytes respond to endoplasmic reticulum (ER) stress with decreased expression of extracellular matrix proteins, and prolonged ER stress leads to chondrocyte apoptosis. Here we demonstrate for the first time that BAG‐1 is involved in ER stress‐induced apoptosis in chondrocytes. Induction of ER stress through multiple mechanisms all resulted in downregulation of BAG‐1 expression. In addition, direct suppression of BAG‐1 expression resulted in chondrocyte growth arrest and apoptosis, while stable overexpression of BAG‐1 delayed the onset of ER stress‐mediated apoptosis. In addition to regulating apoptosis, we also observed decreased expression of collagen type II in BAG‐1 deficient chondrocytes. In contrast, overexpression of BAG‐1 resulted in increased expression of collagen type II. Moreover, under ER stress conditions, the reduced expression of collagen type II was delayed in chondrocytes overexpressing BAG‐1. These results suggest a novel role for BAG‐1 in supporting viability and matrix expression of chondrocytes. J. Cell. Biochem. 102: 786–800, 2007. © 2007 Wiley‐Liss, Inc.
Details
- Title: Subtitle
- A novel role for Bcl‐2 associated‐athanogene‐1 (Bag‐1) in regulation of the endoplasmic reticulum stress response in mammalian chondrocytes
- Creators
- Ling YangDenise McBurneyShou‐Ching TangSara G CarlsonWalter E Horton
- Resource Type
- Journal article
- Publication Details
- Journal of cellular biochemistry, Vol.102(3), pp.786-800
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- DOI
- 10.1002/jcb.21328
- PMID
- 17546604
- ISSN
- 0730-2312
- eISSN
- 1097-4644
- Number of pages
- 15
- Language
- English
- Date published
- 10/15/2007
- Academic Unit
- Molecular Physiology and Biophysics; Anatomy and Cell Biology
- Record Identifier
- 9984025478202771
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