Journal article
A novel trafficking signal within the HLA-C cytoplasmic tail allows regulated expression upon differentiation of macrophages
The Journal of immunology (1950), Vol.180(12), pp.7804-7817
06/15/2008
DOI: 10.4049/jimmunol.180.12.7804
PMCID: PMC2440697
PMID: 18523244
Abstract
MHC class I molecules (MHC-I) present peptides to CTLs. In addition, HLA-C allotypes are recognized by killer cell Ig-like receptors (KIR) found on NK cells and effector CTLs. Compared with other classical MHC-I allotypes, HLA-C has low cell surface expression and an altered intracellular trafficking pattern. We present evidence that this results from effects of both the extracellular domain and the cytoplasmic tail. Notably, we demonstrate that the cytoplasmic tail contains a dihydrophobic (LI) internalization and lysosomal targeting signal that is partially attenuated by an aspartic acid residue (DXSLI). In addition, we provide evidence that this signal is specifically inhibited by hypophosphorylation of the adjacent serine residue upon macrophage differentiation and that this allows high HLA-C expression in this cell type. We propose that tightly regulated HLA-C surface expression facilitates immune surveillance and allows HLA-C to serve a specialized role in macrophages.
Details
- Title: Subtitle
- A novel trafficking signal within the HLA-C cytoplasmic tail allows regulated expression upon differentiation of macrophages
- Creators
- Malinda R. Schaefer - Univ Michigan, Grad Program Immunol, Ann Arbor, MI 48109 USAMaya Williams - Univ Michigan, Grad Program Cellular & Mol Biol, Ann Arbor, MI 48109 USADeanna A. Kulpa - Univ Michigan, Dept Med, Ann Arbor, MI 48109 USAPennelope K. Blakely - Univ Michigan, Dept Med, Ann Arbor, MI 48109 USAAnna Q. Yaffee - University of MichiganKathleen L. Collins - University of Michigan
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.180(12), pp.7804-7817
- DOI
- 10.4049/jimmunol.180.12.7804
- PMID
- 18523244
- PMCID
- PMC2440697
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- Amer Assoc Immunologists
- Number of pages
- 14
- Grant note
- R01 AI051192-05; AI 046998; R01 AI046998; R01 AI051192; R01 AI046998-09; R01 AI 051198 / NIAID NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) R01AI046998 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID)
- Language
- English
- Date published
- 06/15/2008
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9984697044202771
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