A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans

Morgann C Reilly; Steven B Levery; Sherry A Castle; J Stacey Klutts; Tamara L Doering

doi:10.1074/jbc.M109.056226

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A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans

Journal article

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A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans

Morgann C Reilly, Steven B Levery, Sherry A Castle, J Stacey Klutts and Tamara L Doering

The Journal of biological chemistry, Vol.284(52), pp.36118-36127

12/25/2009

DOI: 10.1074/jbc.M109.056226

PMCID: PMC2794727

PMID: 19864415

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Abstract

Cryptococcus neoformans is a fungal pathogen that causes serious disease in immunocompromised individuals. The organism produces a distinctive polysaccharide capsule that is necessary for its virulence, a predominantly polysaccharide cell wall, and a variety of protein- and lipid-linked glycans. The glycan synthetic pathways of this pathogen are of great interest. Here we report the detection of a novel glycosylphosphotransferase activity in C. neoformans, identification of the corresponding gene, and characterization of the encoded protein. The observed activity is specific for UDP-xylose as a donor and for mannose acceptors and forms a xylose-alpha-1-phosphate-6-mannose linkage. This is the first report of a xylosylphosphotransferase activity in any system.

Amino Acid Sequence

Xylose - metabolism

Cryptococcus neoformans - enzymology

Mannose - genetics

Cryptococcus neoformans - genetics

Cell Wall - genetics

Molecular Sequence Data

Uridine Diphosphate - genetics

Phosphotransferases (Alcohol Group Acceptor) - genetics

Cell Wall - enzymology

Fungal Proteins - genetics

Uridine Diphosphate - metabolism

Xylose - genetics

Phosphotransferases (Alcohol Group Acceptor) - metabolism

Mannose - metabolism

Cryptococcosis - genetics

Carbohydrate Conformation

Cryptococcosis - enzymology

Fungal Proteins - metabolism

Details

Title: Subtitle: A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans
Creators: Morgann C Reilly - Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA
Steven B Levery
Sherry A Castle
J Stacey Klutts
Tamara L Doering
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.284(52), pp.36118-36127
DOI: 10.1074/jbc.M109.056226
PMID: 19864415
PMCID: PMC2794727
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: United States
Grant note: R01 GM071007 / NIGMS NIH HHS
Language: English
Date published: 12/25/2009
Academic Unit: Pathology
Record Identifier: 9984047698902771

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