A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs

Robert A Philibert; Brandan Penaluna; Teresa White; Sarah Shires; Tracy Gunter; Jill Liesveld; Cheryl Erwin; Nancy Hollenbeck; Terry Osborn

doi:10.4161/epi.32252

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A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs

Journal article

Open access

Peer reviewed

A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs

Robert A Philibert, Brandan Penaluna, Teresa White, Sarah Shires, Tracy Gunter, Jill Liesveld, Cheryl Erwin, Nancy Hollenbeck and Terry Osborn

Epigenetics, Vol.9(9), pp.1212-1219

09/2014

DOI: 10.4161/epi.32252

PMCID: PMC4169013

PMID: 25147915

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Abstract

Alcoholism has a profound impact on millions of people throughout the world. However, the ability to determine if a patient needs treatment is hindered by reliance on self-reporting and the clinician's capability to monitor the patient's response to treatment is challenged by the lack of reliable biomarkers. Using a genome-wide approach, we have previously shown that chronic alcohol use is associated with methylation changes in DNA from human cell lines. In this pilot study, we now examine DNA methylation in peripheral mononuclear cell DNA gathered from subjects as they enter and leave short-term alcohol treatment. When compared with abstinent controls, subjects with heavy alcohol use show widespread changes in DNA methylation that have a tendency to reverse with abstinence. Pathway analysis demonstrates that these changes map to gene networks involved in apoptosis. There is no significant overlap of the alcohol signature with the methylation signature previously derived for smoking. We conclude that DNA methylation may have future clinical utility in assessing acute alcohol use status and monitoring treatment response.

Leukocytes, Mononuclear - metabolism

Humans

Middle Aged

Male

Alcohol Abstinence

Case-Control Studies

DNA Methylation

Pilot Projects

Alcoholism - metabolism

Adult

Female

Genome, Human

Alcoholism - therapy

Details

Title: Subtitle: A pilot examination of the genome-wide DNA methylation signatures of subjects entering and exiting short-term alcohol dependence treatment programs
Creators: Robert A Philibert - Behavioral Diagnostics; Iowa City, IA USA; Department of Psychiatry; University of Iowa; Iowa City, IA USA
Brandan Penaluna - Department of Psychiatry; University of Iowa; Iowa City, IA USA
Teresa White - Department of Psychiatry; University of Iowa; Iowa City, IA USA
Sarah Shires - Department of Psychiatry; University of Iowa; Iowa City, IA USA
Tracy Gunter - Department of Psychiatry; Indiana University School of Medicine; Indianapolis, IN USA
Jill Liesveld - Department of Psychiatry; University of Iowa; Iowa City, IA USA
Cheryl Erwin - Departments of Medical Education and Psychiatry; Texas Tech University Health Sciences Center; Lubbock, TX USA
Nancy Hollenbeck - Department of Psychiatry; University of Iowa; Iowa City, IA USA
Terry Osborn - Behavioral Diagnostics; Iowa City, IA USA
Resource Type: Journal article
Publication Details: Epigenetics, Vol.9(9), pp.1212-1219
DOI: 10.4161/epi.32252
PMID: 25147915
PMCID: PMC4169013
NLM abbreviation: Epigenetics
ISSN: 1559-2294
eISSN: 1559-2308
Publisher: United States
Grant note: R43AA022041 / NIAAA NIH HHS P30 DA027827 / NIDA NIH HHS R43 AA022041 / NIAAA NIH HHS
Language: English
Date published: 09/2014
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Psychiatry; Iowa Neuroscience Institute
Record Identifier: 9984003474902771

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