Journal article
A pilot investigation on serum protein fingerprinting of nasopharyngeal carcinoma
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery, Vol.46(6), pp.509-512
06/01/2011
DOI: 10.3760/cma.j.issn.1673-0860.2011.06.015
PMID: 21924105
Abstract
Objective: To establish a preliminary foundation for developing a serum proteomics diagnostic model of nasopharyngeal carcinoma (NPC) by comparing the differences in serum protein fingerprints between patients with NPC and healthy subjects and between different types of NPC.
Methods: The serum samples of 41 patients with different types of NPC and 20 healthy subjects were collected. Surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) were used to detect the blood samples to obtain serum protein mass spectrum, i.e. serum protein fingerprinting. Biomarker Wizard and Biomarker Patterns system software were used to compare the differences in serum protein mass spectrum between NPC patients and healthy subjects and between different types of NPC, and to screen out the NPC-related serum proteins.
Results: Compared with the healthy control, NPC patients emerged 9 very prominent protein peaks (P < 0.001), with the combined differential peaks. No significant difference was found in relative amount of serum proteins with different molecular mass between different types of NPC (P > 0.05).
Conclusions: The serum marker proteins and specific protein fingerprints of NPC can be screened out by SELDI-TOF-MS, which could be used to develop a serum proteomics model for clinical screening and early diagnosis of NPC.
Details
- Title: Subtitle
- A pilot investigation on serum protein fingerprinting of nasopharyngeal carcinoma
- Creators
- Xiao-wen HuangWei ZhouJin ChenHan-qi ChuCai-wen XiaoXiao-yang HuaPing ZhangHui-qin LiYong-hua Cui
- Resource Type
- Journal article
- Publication Details
- Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery, Vol.46(6), pp.509-512
- DOI
- 10.3760/cma.j.issn.1673-0860.2011.06.015
- PMID
- 21924105
- ISSN
- 1673-0860
- Language
- Chinese
- Date published
- 06/01/2011
- Academic Unit
- Otolaryngology
- Record Identifier
- 9984312249602771
Metrics
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