Journal article
A porphyrin-PEG polymer with rapid renal clearance
Biomaterials, Vol.76, pp.25-32
01/01/2016
DOI: 10.1016/j.biomaterials.2015.10.049
PMCID: PMC4662896
PMID: 26517562
Abstract
Tetracarboxylic porphyrins and polyethylene glycol (PEG) diamines were crosslinked in conditions that gave rise to a water-soluble porphyrin polyamide. Using PEG linkers 2 kDa or larger prevented fluorescence self-quenching. This networked porphyrin mesh was retained during dialysis with membranes with a 100 kDa pore size, yet passed through the membrane when centrifugal filtration was applied. Following intravenous administration, the porphyrin mesh, but not the free porphyrin, was rapidly cleared via renal excretion. The process could be monitored by fluorescence analysis of collected urine, with minimal background due to the large Stokes shift of the porphyrin (230 nm separating excitation and emission peaks). In a rhabdomyolysis mouse model of renal failure, porphyrin mesh urinary clearance was significantly impaired. This led to slower accumulation in the bladder, which could be visualized non-invasively via fluorescence imaging. Without further modification, the porphyrin mesh was chelated with Cu-64 for dynamic whole body positron emission tomography imaging of renal clearance. Together, these data show that small porphyrin-PEG polymers can serve as effective multimodal markers of renal function. (c) 2015 Elsevier Ltd. All rights reserved.
Details
- Title: Subtitle
- A porphyrin-PEG polymer with rapid renal clearance
- Creators
- Haoyuan Huang - University at Buffalo, State University of New YorkReinier Hernandez - University of Wisconsin–MadisonJumin Geng - University at Buffalo, State University of New YorkHaotian Sun - University at Buffalo, State University of New YorkWentao Song - University at Buffalo, State University of New YorkFeng Chen - University of Wisconsin–MadisonStephen A. Graves - University of Wisconsin–MadisonRobert J. Nickles - University of Wisconsin–MadisonChong Cheng - University at Buffalo, State University of New YorkWeibo Cai - University of Wisconsin–MadisonJonathan F. Lovell - University at Buffalo, State University of New York
- Resource Type
- Journal article
- Publication Details
- Biomaterials, Vol.76, pp.25-32
- DOI
- 10.1016/j.biomaterials.2015.10.049
- PMID
- 26517562
- PMCID
- PMC4662896
- NLM abbreviation
- Biomaterials
- ISSN
- 0142-9612
- eISSN
- 1878-5905
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- T32GM008349 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) DP5OD017898 / OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01CA169365 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) 1R01CA169365; DP5OD017898 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 01/01/2016
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Radiation Oncology
- Record Identifier
- 9984383910702771
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