Journal article
A positively selecting thymic epithelial cell line lacks costimulatory activity
The Journal of immunology (1950), Vol.152(8), pp.3814-3823
1994
DOI: 10.4049/jimmunol.152.8.3814
PMID: 7511660
Abstract
The participation of costimulatory molecule interactions in positive selection of T lymphocytes was addressed by assessing the ability of a positively selecting thymic epithelial cell (TEC) line, 427.1, to stimulate allospecific CTL responses. Stimulation of H-2s spleen cells with the H-2b expressing 427.1 line does not result in the generation of cells capable of lysing H-2b target cell lines. The level of expression of MHC class I molecules by 427.1 is lower than that found in other stimulatory TEC lines. However, this finding does not account for the nonstimulatory phenotype. Up-regulation of MHC class I did not result in stimulation, and fusion of 427.1 cells with stimulatory TEC resulted in a line with low MHC class I molecule expression and stimulatory phenotype. The TEC line 427.1 does not express the costimulatory molecule B7/BB1, and transfection of the B7/BB1-encoding DNA results in expression of the molecule and conversion into a stimulatory phenotype, demonstrating directly that the non-stimulatory phenotype is a result of lack of costimulation. However, B7/BB1 expression does not improve the ability of 427.1 TECs to induce positive selection. Intrathymic injection of the B7/BB1 transfected, compared with mock transfected 427.1 cells, rescued fewer CD8+ mature thymocytes in beta 2-microglobulin negative mice. Therefore, unlike the peripheral T cell responses to Ag, positive selection of T cells in the thymus may not depend on the costimulation provided by the presenting cell.
Details
- Title: Subtitle
- A positively selecting thymic epithelial cell line lacks costimulatory activity
- Creators
- S VUKMANOVIC - Univ. Washington, dep. immunology, Seattle WA 98195, United StatesG STELLA - Univ. Washington, dep. immunology, Seattle WA 98195, United StatesP. D KING - Univ. Washington, dep. immunology, Seattle WA 98195, United StatesR DYALL - Univ. Washington, dep. immunology, Seattle WA 98195, United StatesK. A HOGQUIST - Univ. Washington, dep. immunology, Seattle WA 98195, United StatesJ. T HARTY - Univ. Washington, dep. immunology, Seattle WA 98195, United StatesJ NIKOLIC-ZUGIC - Univ. Washington, dep. immunology, Seattle WA 98195, United StatesM. J BEVAN - Univ. Washington, dep. immunology, Seattle WA 98195, United States
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.152(8), pp.3814-3823
- Publisher
- American Association of Immunologists; Bethesda, MD
- DOI
- 10.4049/jimmunol.152.8.3814
- PMID
- 7511660
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 1994
- Academic Unit
- Pathology
- Record Identifier
- 9984047981802771
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